
The human IL-1α ELISABASIC kit is ideal for users who want the flexibility of setting up their own ELISA assay. This product enables quantification of human IL-1α in solution, e.g. in cell culture supernatant. The kit contains a matched pair of monoclonal capture and detection antibodies, Streptavidin-ALP, ELISA standard and standard reconstitution buffer. Plates and substrate are not included.
The assay is specific for human IL-1α (pro-form and active form) and shows no cross-reactivity with recombinant human IL-1β or IL-18.
Human IL-1α ELISABASIC kits are also available with Streptavidin-HRP. See Related products.
ELISA substrateReady-to-use ELISA substrate is available in our web shop. See Related products.
Analyte Information
IL-1αInterleukin-1α (IL-1α, also known as hematopoietin 1, Interleukin-1alpha, IL-1 alpha, IL1α and Interleukin-1 alpha) is a proinflammatory cytokine and inducer of acute phase responses. IL-1α is produced primarily by monocytes, macrophages, and neutrophil after induction by bacterial product such as LPS.
Ordering InformationOrders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.
Email:Send your order to order@mabtech.com.
Customers from USA or Canada: Please send your order to mabtech.usa@mabtech.com.Application: |
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Reactivity: |
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Standard range: | 2-200 pg/ml |
Documentation: | Datasheet/ProtocolSDS |
Contents: |
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Size: | for 20 plates |
Other formats: | Click to view all Human IL-1α products |
Very easy to use ELISA kit. |
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尽量使用已经用荧光素标记好的单克隆抗体。
如果是单色实验,荧光素的亮度越强越好,比如标记了APC的抗体就要比标记了pacific blue的在相同抗原量,抗体用量相同的情况下,要亮很多
如果是多色实验,除了不要使用光谱重叠度高的荧光素以外,还要搭配染色指数。简单的说,就是用亮度强的荧光素标记的抗体来染水平低的抗原,而用较弱的荧光素标记的抗体来染高表达的抗原。
英 [ˈfɔ:mæt]美 [ˈfɔ:rmæt]
n. (出版物的)版式;[自](数据安排的)形式;电视节目的总安排(或计划)
vt. 使格式化;安排…的格局;设计…的版面
vi. 设计一个版式
如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
楼上两位还是要仔细看下产品说明书

