The human IL-1α ELISABASIC kit is ideal for users who want the flexibility of setting up their own ELISA assay. This product enables quantification of human IL-1α in solution, e.g. in cell culture supernatant. The kit contains a matched pair of monoclonal capture and detection antibodies, Streptavidin-HRP, ELISA standard and standard reconstitution buffer. Plates and substrate are not included.
The assay is specific for human IL-1α (pro-form and active form) and shows no cross-reactivity with recombinant human IL-1β or IL-18.
Human IL-1α ELISABASIC kits are also available with Streptavidin-ALP. See Related products.
ELISA substrateReady-to-use ELISA substrate is available in our web shop. See Related products.
Analyte Information
IL-1αInterleukin-1α (IL-1α, also known as hematopoietin 1, Interleukin-1alpha, IL-1 alpha, IL1α and Interleukin-1 alpha) is a proinflammatory cytokine and inducer of acute phase responses. IL-1α is produced primarily by monocytes, macrophages, and neutrophil after induction by bacterial product such as LPS.
Ordering InformationOrders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.
Email:Send your order to order@mabtech.com.
Customers from USA or Canada: Please send your order to mabtech.usa@mabtech.com.| Application: |
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| Standard range: | 2-200 pg/ml |
| Documentation: | Datasheet/ProtocolSDS |
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| Size: | for 20 plates |
| Other formats: | Click to view all Human IL-1α products |
Very easy to use ELISA kit. | |
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如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
利用CD3/CD4/IL-17的抗体组合(不同荧光素标记),具体步骤可按照淋巴细胞TH1/2亚型分析的protocol!
一般都是先做CD4的surface staining
然后再做IFN-gamma和IL-17的intrecellular staining
测流式后不用水洗
不用问为什么?_?
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