The human Interleukin-3 (IL-3) ELISABASIC kit is ideal for users who want the flexibility of setting up their own ELISA assay. This product enables quantification of human IL-3 in solution, e.g. in cell culture supernatant. The kit contains a matched pair of monoclonal capture and detection antibodies, Streptavidin-ALP, ELISA standard and standard reconstitution buffer. Plates and substrate are not included.
Human IL-3 ELISABASIC kits are also available with Streptavidin-HRP. See Related products.
Serum/Plasma samplesAnalysis of serum/plasma samples with this kit requires the use of ELISA diluent for dilution of the samples, standard and detection antibody. The diluent prevents false positive read-outs which may be caused by interference of heterophilic antibodies found in serum and plasma. The ELISA diluent is available from Mabtech. See Related products.
ELISA substrateReady-to-use ELISA substrate is available in our web shop. See Related products.
Analyte Information
IL-3The cytokine Interleukin-3 (IL-3, IL3) is mainly produced by activated T cells. IL-3 is also called multi-lineage-colony stimulating factor since it induces the growth and differentiation of many cells. IL-3 is also an activator of mast cells, eosinophils, basophils and macrophages.
Ordering InformationOrders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.
Email:Send your order to order@mabtech.com.
Customers from USA or Canada: Please send your order to mabtech.usa@mabtech.com.| Application: |
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| Standard range: | 25-2500 pg/ml |
| Documentation: | Datasheet/ProtocolSDS |
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| Size: | for 6 plates |
| Other formats: | Click to view all Human IL-3 products |
Very easy to use ELISA kit. | |
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如题,试剂商推荐这家的,然后网上查了一下就是武汉三鹰啊,国产的,有点虚啊?请前辈指点迷津啊
尽量使用已经用荧光素标记好的单克隆抗体。
如果是单色实验,荧光素的亮度越强越好,比如标记了APC的抗体就要比标记了pacific blue的在相同抗原量,抗体用量相同的情况下,要亮很多
如果是多色实验,除了不要使用光谱重叠度高的荧光素以外,还要搭配染色指数。简单的说,就是用亮度强的荧光素标记的抗体来染水平低的抗原,而用较弱的荧光素标记的抗体来染高表达的抗原。
高保真生物技术有限公司~~实验整体外包服务商~~~
anti-mouse TCR γ/δ Antibody
anti-human TCR γ/δ Antibody
利用CD3/CD4/IL-17的抗体组合(不同荧光素标记),具体步骤可按照淋巴细胞TH1/2亚型分析的protocol!
一般都是先做CD4的surface staining
然后再做IFN-gamma和IL-17的intrecellular staining

