The single-analyte FluoroSpot assay provides an ELISpot alternative with fluorescent detection instead of enzymatic. The assay enables enumeration of cells secreting human Interferon-gamma (IFN-γ).

The kit includes low fluorescent plates pre-coated with monoclonal capture antibody, monoclonal detection antibody, secondary reagent conjugated to fluorophore, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. A polyclonal activator for human T cells (mAb CD3-2) is supplied as a positive control for cytokine secretion.

A FluoroSpot reader equipped with a bandpass filter for excitation at 490 nm and emission at 510 nm (e.g., LED490, FITC-equivalent) is recommended for analysis.

IFN-γInterferon-γ (IFN-γ, also known as IFN-gamma, IFN-g, IFNg and Interferon-gamma) is produced mainly by activated T cells and NK cells. It is a proinflammatory cytokine that activates macrophages and endothelial cells, but it also regulates immune responses by effecting APC and T and B cells. Production of IFN-γ by helper T cells as well as cytotoxic T cells is a hallmark of the TH1-type phenotype, thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Orders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.

Email:Send your order toorder@mabtech.com.