ThismonoclonalantibodyenablessensitiveandspecificdetectionofguineapigIFN-γproducingcellsinflowcytometry.
Ready-to-usesolutionofPF488P-conjugatedmAbMT4A27.
PF488Pisexcitedbythebluelaser(488nm).Theexcitationmaxis490nmandtheemissionmaxis516nm.
AnalyteInformation
IFN-γInterferon-γ(IFN-γ,alsoknownasIFN-gamma,IFN-g,IFNgandInterferon-gamma)isproducedmainlybyactivatedTcellsandNKcells.Itisaproinflammatorycytokinethatactivatesmacrophagesandendothelialcells,butitalsoregulatesimmuneresponsesbyeffectingAPCandTandBcells.ProductionofIFN-γbyhelperTcellsaswellascytotoxicTcellsisahallmarkoftheTH1-typephenotype,thus,high-levelproductionofIFN-γistypicallyassociatedwitheffectivehostdefenseagainstintracellularpathogens.
OrderingInformationOrderstoMabtechcanbeplacedonline,byemail,orbyphone/faxtoanyofouroffices.
Email:Sendyourordertoorder@mabtech.com.
CustomersfromUSAorCanada:Pleasesendyourordertomabtech.usa@mabtech.com.| Application: |
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| Reactivity: |
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| Format: | Fluorophore-conjugated |
| Specificity: | NativeandrecombinantIFN-γ |
| Isotype: | MouseIgG1 |
| Documentation: | DatasheetSDS |
| Contents: |
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| Size: | 100tests |
| Otherformats: | ClicktoviewallGuineapigIFN-γproducts |
ItrulybelievethatMabtechsellshigh-qualityandreliabletoolstoconductourresearch.Moreover,theyhaveaverygoodtechnicalandscientificsupport.Theyareveryaccommodatingtohelpustotesttheirproductsandtodevelopnewtechnologiesinthelab. | |
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请问一下做流式需要的抗体那种比较好,有人说Biolegend,eBioscience,santa,BD。求各位大神指教
如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
楼上两位还是要仔细看下产品说明书
多抗虽然可能可以产生更强的信号,但是特异性差,染色后荧光强度和抗原水平不是线性关系,不同批次差异很大。所以流式检测不使用多抗。

