This monoclonal antibody enables sensitive and specific detection of IL-4 producing cells in flow cytometry.
Ready-to-use solution of PF648P-conjugated mAb IL4-3.
PF647P is excited by the red laser (633 nm). The excitation max is 654 nm and the emission max is 672 nm.
Species cross-reactivityThe monoclonal antibody cross-reacts with IL-4 from non-human primates. See the Human/NHP cross-reactivity table.
Analyte Information
IL-4Interleukin-4 (IL-4, IL4) is produced by activated T cells, especially differentiated CD4+ TH2-type cells, and stimulated mast cells and basophils. The most important effects of IL-4 are the regulation of helper T cell differentiation to the TH2 type and the regulation of IgE and IgG4 production by B cells.
Ordering InformationOrders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.
Email:Send your order toorder@mabtech.com.
Customers from USA or Canada: Please send your order tomabtech.usa@mabtech.com.| Application: |
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| Format: | Fluorophore-conjugated |
| Specificity: | Native IL-4 |
| Isotype: | Rat IgG1 |
| Documentation: | DatasheetSDS |
| Contents: |
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| Size: | 100 tests |
| Other formats: | Click to view all Human/NHP IL-4 products |
I truly believe that Mabtech sells high-quality and reliable tools to conduct our research. Moreover, they have a very good technical and scientific support. They are very accommodating to help us to test their products and to develop new technologies in the lab. | |
ebiomall.com
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请问一下做流式需要的抗体那种比较好,有人说Biolegend,eBioscience,santa,BD。求各位大神指教
如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
楼上两位还是要仔细看下产品说明书
多抗虽然可能可以产生更强的信号,但是特异性差,染色后荧光强度和抗原水平不是线性关系,不同批次差异很大。所以流式检测不使用多抗。

