The human high sensitivity IgG ELISA development kit is ideal for users who want the flexibility of setting up their own ELISA assay. This product enables quantification of total human IgG in solution, e.g. in serum, plasma and cell culture supernatants, and contains matched quantities of monoclonal capture antibody, biotinylated detection antibody, Streptavidin-ALP and ELISA standard. Plates and substrate are not included.

The monoclonal antibodies in this kit are specific for the Fc part of human IgG.

Analysis of serum/plasma samples diluted less than 10 times in the assay requires the use of ELISA diluent for dilution of the samples, standard and detection antibody. The diluent prevents false positive read-outs which may be caused by interference of heterophilic antibodies found in serum and plasma. The ELISA diluent is available from Mabtech. See Related products.

The monoclonal antibodies in this kit cross-react with IgG from non-human primates. See the Human/NHP cross-reactivity table.

Ready-to-use ELISA substrate is available in our web shop. See Related products.

IgGIgG (Immunoglobulin G) is the most abundant Ig isotype in serum and makes up approximately 80% of all serum Immunoglobulins. In humans, there are four subclasses of IgG with highest serum concentrations of IgG1 followed by IgG2, IgG3 and IgG4. In mice the IgG subclasses are defined IgG1, IgG2a/c, IgG2b and IgG3. The IgG molecule consists of two heavy and two lights chains (κ or λ) resulting in a molecule with two arms for antigen binding. High levels of IgG antibodies are induced following the initial IgM response in a typical immune response to antigens.

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Email:Send your order toorder@mabtech.com.