Thesingle-analyteFluoroSpotassayprovidesanELISpotalternativewithfluorescentdetectioninsteadofenzymatic.TheassayenablesenumerationofcellssecretingmonkeyInterferon-gamma(IFN-γ).
Thekitincludeslowfluorescentplatespre-coatedwithmonoclonalcaptureantibody,monoclonaldetectionantibody,secondaryreagentconjugatedtofluorophore,afluorescenceenhancerandananti-CD28monoclonalantibodyforco-stimulation.Theincludedanti-CD3mAbcanbeusedtoinducecytokinesecretionandisdependentonnumber,natureandviABIlityofaccessorycells.Theantibodycross-reactswithCD3oncynomolgusandrhesusmacaqueTcells.
AFluoroSpotreaderequippedwithabandpassfilterforexcitationat550nmandemissionat570nm(e.g.,LED550,Cy3-equivalent)isrecommendedforanalysis.
Speciescross-reactivityThemonoclonalantibodiesinthiskitreactwithhumanIFN-γandcross-reactwithIFN-γfromnon-humanprimates.SeetheHuman/NHPcross-reactivitytable.
AnalyteInformation
IFN-γInterferon-γ(IFN-γ,alsoknownasIFN-gamma,IFN-g,IFNgandInterferon-gamma)isproducedmainlybyactivatedTcellsandNKcells.Itisaproinflammatorycytokinethatactivatesmacrophagesandendothelialcells,butitalsoregulatesimmuneresponsesbyeffectingAPCandTandBcells.ProductionofIFN-γbyhelperTcellsaswellascytotoxicTcellsisahallmarkoftheTH1-typephenotype,thus,high-levelproductionofIFN-γistypicallyassociatedwitheffectivehostdefenseagainstintracellularpathogens.
OrderingInformationOrderstoMabtechcanbeplacedonline,byemail,orbyphone/faxtoanyofouroffices.
Email:Sendyourordertoorder@mabtech.com.
CustomersfromUSAorCanada:Pleasesendyourordertomabtech.usa@mabtech.com.| Application: |
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| Format: | FluoroSpotPLUS |
| Documentation: | Datasheet/ProtocolSDS |
| Contents: |
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| Size: | 2plates |
| Otherformats: | ClicktoviewallNHPIFN-γproducts |
WehaveusedMabtechELISPOTantibodiesandreagentsforseveralyears.Thehighqualityandstandardandcompetitivepricewillkeepushookedforyearstocome.RecentlywehavestartedworkingwithFluorospot,whichhasreallyhelpedusmoveforwardandhaveopenednewavenuesinourresearch.Asalwaysmanagementandtechnicalstaffhaveallprovidedimpeccableservice. | |
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请问一下做流式需要的抗体那种比较好,有人说Biolegend,eBioscience,santa,BD。求各位大神指教
如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
楼上两位还是要仔细看下产品说明书
多抗虽然可能可以产生更强的信号,但是特异性差,染色后荧光强度和抗原水平不是线性关系,不同批次差异很大。所以流式检测不使用多抗。

