The single-analyte FluoroSpot assay provides an ELISpot alternative with fluorescent detection instead of enzymatic. The assay enables enumeration of cells secreting mouse Interferon-gamma (IFN-γ).
The kit includes low fluorescent plates pre-coated with monoclonal capture antibody, monoclonal detection antibody, secondary reagent conjugated to fluorophore, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation.
A FluoroSpot reader equipped with a bandpass filter for excitation at 550 nm and emission at 570 nm (e.g., LED550, Cy3-equivalent) is recommended for analysis.
Analyte Information
IFN-γInterferon-γ (IFN-γ, also known as IFN-gamma, IFN-g, IFNg and Interferon-gamma) is produced mainly by activated T cells and NK cells. It is a proinflammatory cytokine that activates macrophages and endothelial cells, but it also regulates immune responses by effecting APC and T and B cells. Production of IFN-γ by helper T cells as well as cytotoxic T cells is a hallmark of the TH1-type phenotype, thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
Ordering InformationOrders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.
Email:Send your order toorder@mabtech.com.
Customers from USA or Canada: Please send your order tomabtech.usa@mabtech.com.| Application: |
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| Format: | FluoroSpotPLUS |
| Documentation: | Datasheet/ProtocolSDS |
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| Size: | 2 plates |
| Other formats: | Click to view all Mouse IFN-γ products |
We have used Mabtech ELISPOT antibodies and reagents for several years. The high quality and standard and competitive price will keep us hooked for years to come. Recently we have started working with Fluorospot, which has really helped us move forward and have opened new avenues in our research. As always management and technical staff have all provided impeccable service. | |
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请问一下做流式需要的抗体那种比较好,有人说Biolegend,eBioscience,santa,BD。求各位大神指教
如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
楼上两位还是要仔细看下产品说明书
多抗虽然可能可以产生更强的信号,但是特异性差,染色后荧光强度和抗原水平不是线性关系,不同批次差异很大。所以流式检测不使用多抗。

