I have compared Mabtech ELISpot assay to ELISpot assays we run at our company on regular basis "head-to-head" using identical samples. Mabtech"s kits proved to be superior to our protocol, had significantly lower background and provided better and higher detection of spots.
Human IgG3 ELISpotBASIC enables enumeration of B cells secreting total IgG3 as well as antigen-specific IgG3. It contains a matched pair of monoclonal capture and detection antibodies, Streptavidin-HRP and polyclonal activators.
With this kit, detection of antigen-specific IgG3 secreting B cells can be performed using coated antigen. If detection with biotinylated antigen is preferred, the plate should instead be coated with the subclass specific antibody, which is available in the web shop. See Related products.
For analysis of memory B cells, we recommend pre-stimulation with R848 and recombinant human IL-2 (both included).
IgG3 ELISpotBASIC is also available with Streptavidin-HRP. See Related products.
As complementary products, we offer ELISpot plates and substrates. See Related products.
Analyte Information
IgG3IgG (Immunoglobulin G) is the most abundant Ig isotype in serum and makes up approximately 80% of all serum Immunoglobulins. In humans, there are four subclasses of IgG with highest serum concentrations of IgG1 followed by IgG2, IgG3 and IgG4. In mice, the IgG subclasses are defined IgG1, IgG2a/c, IgG2b and IgG3. IgG3 binds to Fc receptors with high affinity and has is a good complement activator.
Ordering InformationOrders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.
Email:Send your order toorder@mabtech.com.
Customers from USA or Canada: Please send your order tomabtech.usa@mabtech.com.| Application: |
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| Documentation: | Datasheet/ProtocolSDS |
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| Size: | for 4 plates |
| Other formats: | Click to view all Human IgG3 products |
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如题,试剂商推荐这家的,然后网上查了一下就是武汉三鹰啊,国产的,有点虚啊?请前辈指点迷津啊
尽量使用已经用荧光素标记好的单克隆抗体。
如果是单色实验,荧光素的亮度越强越好,比如标记了APC的抗体就要比标记了pacific blue的在相同抗原量,抗体用量相同的情况下,要亮很多
如果是多色实验,除了不要使用光谱重叠度高的荧光素以外,还要搭配染色指数。简单的说,就是用亮度强的荧光素标记的抗体来染水平低的抗原,而用较弱的荧光素标记的抗体来染高表达的抗原。
高保真生物技术有限公司~~实验整体外包服务商~~~
anti-mouse TCR γ/δ Antibody
anti-human TCR γ/δ Antibody
利用CD3/CD4/IL-17的抗体组合(不同荧光素标记),具体步骤可按照淋巴细胞TH1/2亚型分析的protocol!
一般都是先做CD4的surface staining
然后再做IFN-gamma和IL-17的intrecellular staining

