I have compared Mabtech ELISpot assay to ELISpot assays we run at our company on regular basis "head-to-head" using identical samples. Mabtech"s kits proved to be superior to our protocol, had significantly lower background and provided better and higher detection of spots.
Human IgG4 ELISpotBASIC enables enumeration of B cells secreting total IgG4 as well as antigen-specific IgG4. It contains a matched pair of monoclonal capture and detection antibodies, Streptavidin-ALP and polyclonal activators.
With this kit, detection of antigen-specific IgG4 secreting B cells can be performed using coated antigen. If detection with biotinylated antigen is preferred, the plate should instead be coated with the subclass specific antibody, which is available in the web shop. See Related products.
For memory B cells, we recommend pre-stimulation with R848 and recombinant human IL-2 (both included).
IgG4 ELISpotBASIC is also available with Streptavidin-HRP. See Related products.
As complementary products, we offer ELISpot plates and substrates. See Related products.
Analyte Information
IgG4IgG (Immunoglobulin G) is the most abundant Ig isotype in serum and makes up approximately 80% of all serum Immunoglobulins. In humans, there are four subclasses of IgG with highest serum concentrations of IgG1 followed by IgG2, IgG3 and IgG4. IgG4 cannot activate the complement system but binds well to Fc receptors.
Ordering InformationOrders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.
Email:Send your order toorder@mabtech.com.
Customers from USA or Canada: Please send your order tomabtech.usa@mabtech.com.| Application: |
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| Documentation: | Datasheet/ProtocolSDS |
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| Size: | for 4 plates |
| Other formats: | Click to view all Human IgG4 products |
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请问一下做流式需要的抗体那种比较好,有人说Biolegend,eBioscience,santa,BD。求各位大神指教
如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
楼上两位还是要仔细看下产品说明书
多抗虽然可能可以产生更强的信号,但是特异性差,染色后荧光强度和抗原水平不是线性关系,不同批次差异很大。所以流式检测不使用多抗。

