![Mabtech/Human apoA1 ELISA PRO kit (3710-1HP-1)/3710-1HP-1](images/no_picture.gif)
The apoA1 ELISAPRO kit provides all necessary reagents and solutions required to quantify apoA1 in solution, e.g. in cell culture supernatant, and serum/plasma samples. The kit contains ELISA plates pre-coated with monoclonal capture antibody, biotinylated detection antibody, Streptavidin-HRP, TMB substrate, ELISA standard and Apo ELISA buffer concentrate. The Apo ELISA buffer enables analysis of serum/plasma samples without interference from heterophilic antibodies. The kit contains a pre-coated plate and matched amount of reagents and solutions.
Dilution guidelines for serum/plasma samples can be found here.
Pre-coated ELISA plates save time and reduce assay variability.
Species cross-reactivityThe monoclonal antibodies in this kit cross-react with apoA1 from non-human primates. See the Human/NHP cross-reactivity table.
Analyte Information
ApoA1Apolipoprotein A1 (apoA1, also known as apolipoprotein-A1, apo A1 and apoA-I) is the main protein component of high density lipoprotein particles (HDL). ApoA1 (28 kD) is mainly produced in the liver and exported into the circulation where it acquires lipids and becomes HDL. High level of apoA1 is regarded as protective against atherosclerosis and cardiovascular disease. Normal concentration of apoA1 in human plasma is approximately 0.8-2.2 g/L.
Ordering InformationOrders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.
Email:Send your order toorder@mabtech.com.
Customers from USA or Canada: Please send your order tomabtech.usa@mabtech.com.Application: |
|
---|---|
Reactivity: |
|
Standard range: | 0.63-40 ng/ml |
Documentation: | Datasheet/ProtocolSDS |
Contents: |
|
Size: | 1 plate |
Other formats: | Click to view all Human/NHP apoA1 products |
Very easy to use ELISA kit. |
ebiomall.com
![公司介绍](/themes/default/images/goods_detail/img_introduction.png)
![](/themes/default/images/goods_detail/company_introduction_bg.png)
![](/themes/default/images/goods_detail/bg_real.png)
![](/themes/default/images/goods_detail/bg_delivery.png)
![](/themes/default/images/goods_detail/bg_express.png)
![](/themes/default/images/goods_detail/bg_service.png)
>
>
>
>
>
>
>
>
>
>
>
>
测流式后不用水洗
不用问为什么?_?
比如,你使用了小鼠IgG1, PE 标记的特异性抗体,那就选用小鼠IgG1, PE标记的非特异性抗体。
下面这个图,是一个比较典型的使用了同型对照的结果图向左转|向右转灰色的曲线是未染色的样本,黄色是同型对照,红色是特异性抗体染色。由于细胞对抗体的非特异性结合,一般同型对照都会比未染色样本的荧光信号要强。所以设置阴性阈值的时候,要根据同型对照,而不是未染色的细胞。
如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
楼上两位还是要仔细看下产品说明书
![](/themes/default/images/img_brandintro.png)
![品牌介绍](/data/brandlogo/1504034773977882321.jpg)