The apoB ELISAPRO kit provides all necessary reagents and solutions required to quantify apoB in solution, e.g. in cell culture supernatant, and serum/plasma samples. The assay is specific for apoB100 and does not recognize apoB48. The kit contains ELISA plates pre-coated with monoclonal capture antibody, biotinylated detection antibody, Streptavidin-HRP, TMB substrate, ELISA standard and Apo ELISA buffer concentrate. The Apo ELISA buffer enables analysis of serum/plasma samples without interference from heterophilic antibodies. The kit contains a pre-coated plate and matched amounts of reagents and solutions.
Dilution guidelines for serum/plasma samples can be found here.
Pre-coated ELISA plates save time and reduce assay variability.
Species cross-reactivityThe monoclonal antibodies in this kit cross-react with apoB from non-human primates. See the Human/NHP cross-reactivity table.
Analyte Information
ApoBApolipoprotein B (apoB, also known as apolipoproteinB, apo B, apoB-100, apoB100 and apo B100) is the main protein component of low density lipoprotein particles (LDL) and it is a large water insoluble molecule of 512 kD (apoB-100) produced mainly in the liver and in the intestine (apoB-48). ApoB is regarded as a negative factor in cardiovascular disease. ApoB-100 is normally 98% of the apoB in human plasma and at a concentration of approximately 0.4-1.3 mg/ml.
Ordering InformationOrders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.
Email:Send your order toorder@mabtech.com.
Customers from USA or Canada: Please send your order tomabtech.usa@mabtech.com.| Application: |
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| Standard range: | 7.8-500 ng/ml |
| Documentation: | Datasheet/ProtocolSDS |
| Contents: |
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| Size: | 1 plate |
| Other formats: | Click to view all Human/NHP apoB products |
Very easy to use ELISA kit. | |
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请问一下做流式需要的抗体那种比较好,有人说Biolegend,eBioscience,santa,BD。求各位大神指教
如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
楼上两位还是要仔细看下产品说明书
多抗虽然可能可以产生更强的信号,但是特异性差,染色后荧光强度和抗原水平不是线性关系,不同批次差异很大。所以流式检测不使用多抗。

