The apoE ELISA^PRO kit provides all necessary reagents and solutions required to quantify apoE in solution, e.g. in cell culture supernatant, and serum/plasma samples. The three isoforms apoE2, apoE3 and apoE4 are detected. The kit contains ELISA plates pre-coated with monoclonal capture antibody, biotinylated detection antibody, Streptavidin-HRP, TMB substrate, ELISA standard and Apo ELISA buffer concentrate. The Apo ELISA buffer enables analysis of serum/plasma samples without interference from heterophilic antibodies. The kit contains a pre-coated plate and matched amounts of reagents and solutions.

Dilution guidelines for serum/plasma samples can be found here.

Pre-coated ELISA plates save time and reduce assay variability.

ApoEApolipoprotein E (apoE, also known as apolipoproteinE and apo E) is a 34 kD glycoprotein involved in lipid transport, lipoprotein metabolism and cellular signaling. It is present in plasma lipoprotein particles such as chylomicrons, VLDL and HDL. ApoE is also present in large amounts at sites of neurological damage and appears to be involved in recycling of apoptotic remnants and of amyloidal aggregates. ApoE is mainly produced in the liver and by macrophages, but also by other cell types such as smooth muscle cells or neuronal cells. In human apoE exists in three isoforms, apoE2, apoE3 and apoE4. Of these, apoE4 is associated with Alzheimer’s disease. Normal concentration of apoE in human plasma is approximately 30-40 µg/ml. The concentration of ApoE in inbred mouse plasma can vary between 15-150 µg/ml.

Orders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.

Email:Send your order toorder@mabtech.com.