The apoH ELISAPRO kit provides all necessary reagents and solutions required to quantify apoH in solution, e.g. in cell culture supernatant, and serum/plasma samples. The kit contains an ELISA plate pre-coated with monoclonal capture antibody, biotinylated detection antibody, Streptavidin-HRP, TMB substrate, ELISA standard and Apo ELISA buffer concentrate. The Apo ELISA buffer enables analysis of serum/plasma samples without interference from heterophilic antibodies. The kit contains a pre-coated plate and matched amount of reagents and solutions.
Dilution guidelines for serum/plasma samples can be found here.
Species cross-reactivityThe monoclonal antibodies in this kit cross-react with apoH from non-human primates. See the Human/NHP cross-reactivity table.
Analyte Information
ApoHApolipoprotein H (apoH, also known as apolipoproteinH, apo H, beta 2-glycoprotein 1 and β2-glycoprotein I) is associated to VLDL, HDL and chylomicrons in plasma and binds to anionic lipids e.g. phospholipids. ApoH is the dominant target of autoantibodies in anti-phosphlipid syndrome (APS). In the blood coagulation pathway, apoH is involved both as an anti-coagulant and as a procoagulant. Normal concentration of apoH in human plasma is approximately 0.05-0.6 g/L.
Ordering InformationOrders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.
Email:Send your order toorder@mabtech.com.
Customers from USA or Canada: Please send your order tomabtech.usa@mabtech.com.| Application: |
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| Standard range: | 0.02-15 ng/ml |
| Documentation: | Datasheet/ProtocolSDS |
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| Size: | 1 plate |
| Other formats: | Click to view all Human/NHP apoH products |
Very easy to use ELISA kit. | |
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如题,试剂商推荐这家的,然后网上查了一下就是武汉三鹰啊,国产的,有点虚啊?请前辈指点迷津啊
尽量使用已经用荧光素标记好的单克隆抗体。
如果是单色实验,荧光素的亮度越强越好,比如标记了APC的抗体就要比标记了pacific blue的在相同抗原量,抗体用量相同的情况下,要亮很多
如果是多色实验,除了不要使用光谱重叠度高的荧光素以外,还要搭配染色指数。简单的说,就是用亮度强的荧光素标记的抗体来染水平低的抗原,而用较弱的荧光素标记的抗体来染高表达的抗原。
高保真生物技术有限公司~~实验整体外包服务商~~~
anti-mouse TCR γ/δ Antibody
anti-human TCR γ/δ Antibody
利用CD3/CD4/IL-17的抗体组合(不同荧光素标记),具体步骤可按照淋巴细胞TH1/2亚型分析的protocol!
一般都是先做CD4的surface staining
然后再做IFN-gamma和IL-17的intrecellular staining

