The human IL-6 ELISAPRO kit provides all necessary reagents and solutions required to quantify human IL-6 in solution, e.g. in cell culture supernatant, and serum/plasma samples. The kit contains an ELISA plate pre-coated with monoclonal capture antibody, biotinylated detection antibody, Streptavidin-HRP, TMB substrate, ELISA standard and ELISA diluent. The ELISA diluent prevents false positive read-outs which may be caused by heterophilic antibodies found in serum and plasma samples.
Pre-coated ELISA plates save time and reduce assay variability.
Species cross-reactivityThe monoclonal antibodies in this kit cross-react with IL-6 from non-human primates. See the Human/NHP cross-reactivity table.
Analyte Information
IL-6Interleukin 6 (IL-6, IL6) is a pleiotropic cytokine that is produced by many different cell types and plays a role in a wide range of functions, such as immune responses, acute-phase reactions, and hematopoiesis. Among other things it augments antibody production from activated B cells in vitro.
Ordering InformationOrders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.
Email:Send your order toorder@mabtech.com.
Customers from USA or Canada: Please send your order tomabtech.usa@mabtech.com.| Application: |
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| Standard range: | 10-3160 pg/ml |
| Documentation: | Datasheet/ProtocolSDS |
| Contents: |
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| Size: | 1 plate |
| Other formats: | Click to view all Human/NHP IL-6 products |
Very easy to use ELISA kit. | |
ebiomall.com
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如题,试剂商推荐这家的,然后网上查了一下就是武汉三鹰啊,国产的,有点虚啊?请前辈指点迷津啊
尽量使用已经用荧光素标记好的单克隆抗体。
如果是单色实验,荧光素的亮度越强越好,比如标记了APC的抗体就要比标记了pacific blue的在相同抗原量,抗体用量相同的情况下,要亮很多
如果是多色实验,除了不要使用光谱重叠度高的荧光素以外,还要搭配染色指数。简单的说,就是用亮度强的荧光素标记的抗体来染水平低的抗原,而用较弱的荧光素标记的抗体来染高表达的抗原。
高保真生物技术有限公司~~实验整体外包服务商~~~
anti-mouse TCR γ/δ Antibody
anti-human TCR γ/δ Antibody
利用CD3/CD4/IL-17的抗体组合(不同荧光素标记),具体步骤可按照淋巴细胞TH1/2亚型分析的protocol!
一般都是先做CD4的surface staining
然后再做IFN-gamma和IL-17的intrecellular staining

