The human IL-12/-23 (p40) ELISA^PRO kit provides all necessary reagents and solutions required to quantify human IL-12/-23 (p40) in solution, e.g. in cell culture supernatant, and serum/plasma samples. The kit contains an ELISA plate pre-coated with monoclonal capture antibodies, biotinylated detection antibody, Streptavidin-HRP, TMB substrate, ELISA standard and ELISA diluent. The ELISA diluent prevents false positive read-outs which may be caused by heterophilic antibodies found in serum and plasma samples

The monoclonal antibodies in this kit are specific to the p40 subunit of IL-12 and IL-23. ELISA assays based on antibodies to p40 will detect both IL-12 and IL-23. Such assays can also detect p40 monomers and dimers.

Pre-coated ELISA plates save time and reduce assay variability.

The monoclonal antibodies in this kit cross-react with IL-12/-23 (p40) from non-human primates. See the Human/NHP cross-reactivity table.

IL-12/-23 (p40)Interleukin-12 (IL-12, IL12) is, when biologically active, a heterodimer (p70) consisting of two covalently linked subunits, p35 and p40. IL-12 is produced by antigen-presenting cells, specifically dendritic cells and macrophages. IL-12 is a proinflammatory cytokine that promotes Th1-type responses by inducing IFN-γ production and enhancing proliferation and cytotoxicity of NK and T cells.Interleukin-23 (IL-23) is a disulfide-linked heterodimer which consists of a unique p19 subunit and the p40 subunit of IL-12. IL-23 is a proinflammatory cytokine, which is produced by macrophages and dendritic cells upon infections with certain pathogens. The IL-23 receptor is expressed on Th17 cells and IL-23 is responsible for Th17 expansion and stabilization.Since both IL-12 and IL-23 heterodimers comprise the p40 subunit, capture immunoassays based on antibodies to p40 will detect both cytokines. Such assays can also detect p40 monomers and dimers.

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