ThemonkeyTNF-αELISAdevelopmentkitisidealforuserswhowanttheflexibilityofsettinguptheirownELISAassay.ThisproductenablesquantificationofmonkeyTNF-αinsolution,e.g.incellculturesupernatant.Thekitcontainsamatchedpairofmonoclonalcaptureanddetectionantibodies,Streptavidin-HRPandELISAstandard.Platesandsubstratearenotincluded.
ThemonoclonalantibodiesinthiskitreactwithhumanTNF-αandcross-reactwithTNF-αfromnon-humanprimates.TheELISAstandardsuppliedisrecombinanthumanTNF-α.SeetheHuman/NHPcross-reactivitytable.
MonkeyTNF-αELISAdevelopmentkitsarealsoavailablewithStreptavidin-ALP.
Serum/PlasmasamplesAnalysisofserum/plasmasampleswiththiskitrequirestheuseofELISAdiluentfordilutionofthesamples,standardanddetectionantibody.Thediluentpreventsfalsepositiveread-outswhichmaybecausedbyinterferenceofheterophilicantibodiesfoundinserumandplasma.TheELISAdiluentisavailablefromMabtech.
ELISAsubstrateReady-to-useELISAsubstrateisavailableinourwebshop.SeeRelatedproducts.
AnalyteInformation
TNF-αTumornecrosisfactor-α(TNF-α,alsoknownasTNF-alpha,TNF-a,TNFa,andTumornecrosisfactor-alpha)isproducedbymanydifferentcelltypese.g.monocytes,macrophages,TcellsandBcells.AmongthemanyeffectsofTNF-α,areprotectionagainstbacterialinfection,cellgrowthmodulation,immunesystemregulationandinvolvementinsepticshock.
OrderingInformationOrderstoMabtechcanbeplacedonline,byemail,orbyphone/faxtoanyofouroffices.
Email:Sendyourordertoorder@mabtech.com.
CustomersfromUSAorCanada:Pleasesendyourordertomabtech.usa@mabtech.com.| Application: |
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| Reactivity: |
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| Standardrange: | 4-400pg/ml |
| Documentation: | Datasheet/ProtocolSDS |
| Contents: |
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| Size: | for6plates |
| Otherformats: | ClicktoviewallNHPTNF-αproducts |
| SavetimeandreducevariABIlitywiththecompleteandpre-coatedELISAPROkit | |
VeryeasytouseELISAkit. | |
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如题,试剂商推荐这家的,然后网上查了一下就是武汉三鹰啊,国产的,有点虚啊?请前辈指点迷津啊
尽量使用已经用荧光素标记好的单克隆抗体。
如果是单色实验,荧光素的亮度越强越好,比如标记了APC的抗体就要比标记了pacific blue的在相同抗原量,抗体用量相同的情况下,要亮很多
如果是多色实验,除了不要使用光谱重叠度高的荧光素以外,还要搭配染色指数。简单的说,就是用亮度强的荧光素标记的抗体来染水平低的抗原,而用较弱的荧光素标记的抗体来染高表达的抗原。
高保真生物技术有限公司~~实验整体外包服务商~~~
anti-mouse TCR γ/δ Antibody
anti-human TCR γ/δ Antibody
利用CD3/CD4/IL-17的抗体组合(不同荧光素标记),具体步骤可按照淋巴细胞TH1/2亚型分析的protocol!
一般都是先做CD4的surface staining
然后再做IFN-gamma和IL-17的intrecellular staining

