ThemouseapoEELISAPROkitprovidesallnecessaryreagentsandsolutionsrequiredtoquantifyapoEinsolution,e.g.incellculturesupernatant,andserum/plasmasamples.ThekitcontainsELISAplatespre-coatedwithmonoclonalcaptureantibodyandmatchedquantitiesofbiotinylateddetectionantibody,Streptavidin-HRP,TMBsubstrate,ELISAstandardandbuffers.
Dilutionguidelinesforserum/plasmasamplescanbefoundhere.
Pre-coatedELISAsavestimeandreducesassayvariABIlity.
Standardrange1.56-100ng/ml.Thestandardrangeistherangeinwhichdeterminationsofanalyteconcentrationcanbedonewithprecision,accuracyandlinearity.
SensitivityThelimitofdetectionofthisassayis1.0ng/ml.ItisthelowestconcentrationthatispossIBLetodetectbutnotnecessarilyquantifywithprecisionandaccuracy.
DilutionrecoveryDilutionofserum/plasmasamplesgivesamid-curverecoveryof98-110%inrepeatedexperiments.
PrecisionTheintra-assayvariationis3.7%(CV).Theinter-assayvariationis7.1%(CV).
AnalyteInformation
ApoEApolipoproteinE(apoE,alsoknownasapolipoproteinEandapoE)isa34kDglycoproteininvolvedinlipidtransport,lipoproteinmetabolismandcellularsignaling.Itispresentinplasmalipoproteinparticlessuchaschylomicrons,VLDLandHDL.ApoEisalsopresentinlargeamountsatsitesofneurologicaldamageandappearstobeinvolvedinrecyclingofapoptoticremnantsandofamyloidalaggregates.ApoEismainlyproducedintheliverandbymacrophages,butalsobyothercelltypessuchassmoothmusclecellsorneuronalcells.InhumanapoEexistsinthreeisoforms,apoE2,apoE3andapoE4.Ofthese,apoE4isassociatedwithAlzheimer’sdisease.NormalconcentrationofapoEinhumanplasmaisapproximately30-40µg/ml.TheconcentrationofApoEininbredmouseplasmacanvarybetween15-150µg/ml.
OrderingInformationOrderstoMabtechcanbeplacedonline,byemail,orbyphone/faxtoanyofouroffices.
Email:Sendyourordertoorder@mabtech.com.
CustomersfromUSAorCanada:Pleasesendyourordertomabtech.usa@mabtech.com.| Application: |
|
|---|---|
| Reactivity: |
|
| Standardrange: | 1.56-100ng/ml |
| Documentation: | Datasheet/ProtocolSDS |
| Contents: |
|
| Size: | 10plates |
| Otherformats: | ClicktoviewallMouseapoEproducts |
Overall,IwouldsayyourMouseapoEELISAkitisexcellent. | |
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
如题,试剂商推荐这家的,然后网上查了一下就是武汉三鹰啊,国产的,有点虚啊?请前辈指点迷津啊
尽量使用已经用荧光素标记好的单克隆抗体。
如果是单色实验,荧光素的亮度越强越好,比如标记了APC的抗体就要比标记了pacific blue的在相同抗原量,抗体用量相同的情况下,要亮很多
如果是多色实验,除了不要使用光谱重叠度高的荧光素以外,还要搭配染色指数。简单的说,就是用亮度强的荧光素标记的抗体来染水平低的抗原,而用较弱的荧光素标记的抗体来染高表达的抗原。
高保真生物技术有限公司~~实验整体外包服务商~~~
anti-mouse TCR γ/δ Antibody
anti-human TCR γ/δ Antibody
利用CD3/CD4/IL-17的抗体组合(不同荧光素标记),具体步骤可按照淋巴细胞TH1/2亚型分析的protocol!
一般都是先做CD4的surface staining
然后再做IFN-gamma和IL-17的intrecellular staining

