The bovine IFN-γ ELISAPRO kit provides all necessary reagents and solutions required to quantify bovine IFN-γ in solution, e.g. in cell culture supernatant, and serum/plasma samples. The kit contains an ELISA plate pre-coated with monoclonal capture antibody, biotinylated detection antibody, Streptavidin-HRP, TMB substrate, ELISA standard and ELISA diluent. The ELISA diluent prevents false positive read-outs which may be caused by heterophilic antibodies found in serum and plasma samples.
Pre-coated ELISA plates save time and reduce assay variability.
Species cross-reactivityThe monoclonal antibodies in this kit cross-react with IFN-γ from sheep. See the Veterinary table
Analyte Information
IFN-γInterferon-γ (IFN-γ, also known as IFN-gamma, IFN-g, IFNg and Interferon-gamma) is produced mainly by activated T cells and NK cells. It is a proinflammatory cytokine that activates macrophages and endothelial cells, but it also regulates immune responses by effecting APC and T and B cells. Production of IFN-γ by helper T cells as well as cytotoxic T cells is a hallmark of the TH1-type phenotype, thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
Ordering InformationOrders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.
Email:Send your order toorder@mabtech.com.
Customers from USA or Canada: Please send your order tomabtech.usa@mabtech.com.| Application: |
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| Standard range: | 3.16-1000 pg/ml |
| Documentation: | Datasheet/ProtocolSDS |
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| Size: | 1 plate |
| Other formats: | Click to view all Cow/Sheep IFN-γ products |
Very easy to use ELISA kit. | |
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英 [ˈfɔ:mæt]美 [ˈfɔ:rmæt]
n. (出版物的)版式;[自](数据安排的)形式;电视节目的总安排(或计划)
vt. 使格式化;安排…的格局;设计…的版面
vi. 设计一个版式
如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
流式细胞仪能检测多少个通道
需要同时检测检测多少个指标
厂家的抗体有多少种荧光标记
如果流式细胞仪的通道越多,那么同一份样本能同时做的表面/胞内标志就越多
A、 如何根据流式细胞仪搭配流式抗体
1) BD流式细胞仪
BD流式试剂选择及应用的相关问答与简介
流式细胞仪能检测多少个通道是由有多少根激光决定的以及每根激光的功能决定的,所以首先需要注意两点:
流式细胞仪有哪些激光。比如标配的FACSCalibur只有一根488nm的激光,能做FL1、FL2、FL3三个荧光通道/三个颜色,而选配的Calibur (FACSCalibur的简称)配有488nm和635nm两根激光,可以检测FL1-FL4四个通道/4个颜色。
不同型号的流式细胞仪的同样的一个通道检测荧光素的能力是不一样的,比如Calibur的FL3(第3通道)能检测PE-Texas Red, PE-Cy5, PerCP, PerCPCy5.5,PE-Cy7,而Aria的第3通道只能检测PE-Texas Red, PE-Cy5, PerCP。 Calibur第3通道能检测的PerCP-Cy5.5在FACSAria上是第4通道检测。Calibur和LSR都能检测4个颜色,但是第4个通道检测荧光素是不一样的
能用的条件:1.分子的小鼠和人差异小,2.小鼠细胞本身不含有鼠抗体的结合受体;
不能用的条件:1.分子种属间差异大,2.采用的小鼠细胞株表面有鼠抗体的结合受体。
推荐尽量采用抗鼠的抗体,义翘神州兔单抗技术做出来的FCM抗体不少,可以到官网搜一下看看。

