
This monoclonal antibody enables sensitive and specific detection of human TNF-α in immunoassays such as ELISA and ELISpot.
We recommend MT25C5 as capture antibody in ELISA and ELISpot in combination with biotinylated detection antibody MT20D9. See Related products.
Analyte Information
TNF-αTumor necrosis factor-α (TNF-α, also known as TNF-alpha, TNF-a, TNFa, and Tumor necrosis factor-alpha) is produced by many different cell types e.g. monocytes, macrophages, T cells and B cells. Among the many effects of TNF-α, are protection against bacterial infection, cell growth modulation, immune system regulation and involvement in septic shock.
Ordering InformationOrders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.
Email:Send your order toorder@mabtech.com.
Customers from USA or Canada: Please send your order tomabtech.usa@mabtech.com.Bulk OrdersUpon request, Mabtech"s antibodies are available in unconjugated format and may be purchased in bulk. Please contact Mabtech directly for further details.
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Format: | Purified |
Specificity: | Native and recombinant TNF-α |
Isotype: | Mouse IgG1 |
Documentation: | DatasheetSDS |
Contents: |
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Size: | 250 µg |
Other formats: | Click to view all Human TNF-α products |
I truly believe that Mabtech sells high-quality and reliable tools to conduct our research. Moreover, they have a very good technical and scientific support. They are very accommodating to help us to test their products and to develop new technologies in the lab. |
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如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
楼上两位还是要仔细看下产品说明书
测流式后不用水洗
不用问为什么?_?
请问各位大神,需要做人巨噬细胞的F4/80,CD11b,CD163标记物,用流式,抗体哪家比较好呢,请推荐,谢谢!
如题,试剂商推荐这家的,然后网上查了一下就是武汉三鹰啊,国产的,有点虚啊?请前辈指点迷津啊
能用的条件:1.分子的小鼠和人差异小,2.小鼠细胞本身不含有鼠抗体的结合受体;
不能用的条件:1.分子种属间差异大,2.采用的小鼠细胞株表面有鼠抗体的结合受体。
推荐尽量采用抗鼠的抗体,义翘神州兔单抗技术做出来的FCM抗体不少,可以到官网搜一下看看。

