ThismonoclonalantibodyenablessensitiveandspecificdetectionofmouseIL-1αinimmunoassayssuchasELISAandELISpot.
WerecommendMT951asdetectionantibodyinELISAandELISpotincombinationwithcaptureantibodyMT946.SeeRelatedproducts.
AnalyteInformation
IL-1αInterleukin-1α(IL-1α,alsoknownashematopoietin1,Interleukin-1alpha,IL-1alpha,IL1αandInterleukin-1alpha)isaproinflammatorycytokineandinducerofacutephaseresponses.IL-1αisproducedprimarilybymonocytes,macrophages,andneutrophilafterinductionbybacterialproductsuchasLPS.
OrderingInformationOrderstoMabtechcanbeplacedonline,byemail,orbyphone/faxtoanyofouroffices.
Email:Sendyourordertoorder@mabtech.com.
CustomersfromUSAorCanada:Pleasesendyourordertomabtech.usa@mabtech.com.BulkOrdersUponrequest,Mabtech"santibodiesareavailableinunconjugatedformatandmaybepurchasedinbulk.PleasecontactMabtechdirectlyforfurtherdetails.
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| Format: | Biotinylated |
| Specificity: | NativeandrecombinantIL-1α |
| Isotype: | MouseIgG2a |
| Documentation: | DatasheetSDS |
| Contents: |
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| Size: | 1mg |
| Otherformats: | ClicktoviewallMouseIL-1αproducts |
ItrulybelievethatMabtechsellshigh-qualityandreliabletoolstoconductourresearch.Moreover,theyhaveaverygoodtechnicalandscientificsupport.Theyareveryaccommodatingtohelpustotesttheirproductsandtodevelopnewtechnologiesinthelab. | |
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如题,试剂商推荐这家的,然后网上查了一下就是武汉三鹰啊,国产的,有点虚啊?请前辈指点迷津啊
尽量使用已经用荧光素标记好的单克隆抗体。
如果是单色实验,荧光素的亮度越强越好,比如标记了APC的抗体就要比标记了pacific blue的在相同抗原量,抗体用量相同的情况下,要亮很多
如果是多色实验,除了不要使用光谱重叠度高的荧光素以外,还要搭配染色指数。简单的说,就是用亮度强的荧光素标记的抗体来染水平低的抗原,而用较弱的荧光素标记的抗体来染高表达的抗原。
高保真生物技术有限公司~~实验整体外包服务商~~~
anti-mouse TCR γ/δ Antibody
anti-human TCR γ/δ Antibody
利用CD3/CD4/IL-17的抗体组合(不同荧光素标记),具体步骤可按照淋巴细胞TH1/2亚型分析的protocol!
一般都是先做CD4的surface staining
然后再做IFN-gamma和IL-17的intrecellular staining

