| Product Name | Cat# | Clone# | Isotype | Applications | Reactivity | Unit/Size |
| GFP Monoclonal Antibody | E-AB-20090 | 4A1 | IgG | WB,IP* | GFP | 30μL, 60μL, 120μL, 200μL |
Description
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em).
Spec Sheet E-AB-20090 (PDF File)
| Synonyms | Anti-GFP |
| Concentration | 1 mg/mL |
| Host | Mouse |
| Immunogen | Recombinant Protein |
| Purification Method | Antigen affinity purification |
| Formulation | PBS with 0.02% sodium azide, 50% glycerol, PH7.4 |
| Storage | Store at -20?. Avoid freeze / thaw cycles. |
| Dilution | WB 1:500-1:2000, IP 1:100 |
Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off .
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
* Remark Icon :
WB=Western Blotting, IP=Immunoprecipitation, IF=Immunofluorescence, IHC=Immunohistochemistory, IHC-p=Immunohistochemistory Paraffin, FCM=Flow Cytometry, CH=ChIP Assay
Manufactured by:Elabscience
ebiomall.com
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如果一个抗体可以用来“做western blot，免疫荧光，免疫共沉淀,ELISA”，我估计着是HRP或碱性磷酸酶或生物素之类标记的，然后需要再加荧光标记的二抗，这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光（直接染色），效果还不错，但是反过来就没试过。
利用CD3/CD4/IL-17的抗体组合（不同荧光素标记），具体步骤可按照淋巴细胞TH1/2亚型分析的protocol！
一般都是先做CD4的surface staining
然后再做IFN-gamma和IL-17的intrecellular staining
测流式后不用水洗
不用问为什么?_?
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vi. 设计一个版式
