| Product Name | Cat# | Clone# | Isotype | Applications | Reactivity | Unit/Size |
| GFP Monoclonal Antibody | E-AB-20090 | 4A1 | IgG | WB,IP* | GFP | 30μL, 60μL, 120μL, 200μL |
Description
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em).
Spec Sheet E-AB-20090 (PDF File)
| Synonyms | Anti-GFP |
| Concentration | 1 mg/mL |
| Host | Mouse |
| Immunogen | Recombinant Protein |
| Purification Method | Antigen affinity purification |
| Formulation | PBS with 0.02% sodium azide, 50% glycerol, PH7.4 |
| Storage | Store at -20?. Avoid freeze / thaw cycles. |
| Dilution | WB 1:500-1:2000, IP 1:100 |
Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off .
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
* Remark Icon :
WB=Western Blotting, IP=Immunoprecipitation, IF=Immunofluorescence, IHC=Immunohistochemistory, IHC-p=Immunohistochemistory Paraffin, FCM=Flow Cytometry, CH=ChIP Assay
Manufactured by:Elabscience
ebiomall.com
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如题，试剂商推荐这家的，然后网上查了一下就是武汉三鹰啊，国产的，有点虚啊？请前辈指点迷津啊
尽量使用已经用荧光素标记好的单克隆抗体。
如果是单色实验，荧光素的亮度越强越好，比如标记了APC的抗体就要比标记了pacific blue的在相同抗原量，抗体用量相同的情况下，要亮很多
如果是多色实验，除了不要使用光谱重叠度高的荧光素以外，还要搭配染色指数。简单的说，就是用亮度强的荧光素标记的抗体来染水平低的抗原，而用较弱的荧光素标记的抗体来染高表达的抗原。
高保真生物技术有限公司~~实验整体外包服务商~~~
anti-mouse TCR γ/δ Antibody
anti-human TCR γ/δ Antibody
利用CD3/CD4/IL-17的抗体组合（不同荧光素标记），具体步骤可按照淋巴细胞TH1/2亚型分析的protocol！
一般都是先做CD4的surface staining
然后再做IFN-gamma和IL-17的intrecellular staining
