Retro-X Concentrator provides a fast, simple, and highly efficient method for concentrating any retroviral stock including MMLV- or MSCV-based retroviral supernatants. In the simple protocol, you just mix your retroviral supernatant with the retrovirus concentration reagent, incubate for a short period, and spin the mixture in a standard centrifuge.
You’ll increase your retroviral titer by up to 100-fold and obtain excellent recoveries—with no ultracentrifugation.
Retro-X Concentrator provides a fast, simple, and highly efficient method for concentrating any retroviral stock including MMLV- or MSCV-based retroviral supernatants. In the simple protocol, you just mix your retroviral supernatant with the retrovirus concentration reagent, incubate for a short period, and spin the mixture in a standard centrifuge.
You’ll increase your retroviral titer by up to 100-fold and obtain excellent recoveries—with no ultracentrifugation.
Far simpler than ultracentrifugation
Compared to ultracentrifugation, the Retro-X Concentrator is more flexible in terms of scale, is easier to use than ultracentrifugation, and requires no specialized equipment.
Scalable for supernatants of any volume, any titer
The Retro-X Concentrator reagent is a 4X concentrate, so it can be added to any volume of supernatant containing any amount of virus or any starting titer. At Takara Bio, scalability has been successful for supernatant volumes up to 250 ml.
Increase titers by up to 100 fold
Using the Retro-X Concentrator protocol, we were able to increase the titer of retroviral supernatants for various envelope-pseudotyped retrovirus samples from 46-fold (amphotropic and 10A1 pseudotypes) to 100-fold (ecotropic and VSV-G), when concentrating them from 10 ml down to 100 µl.
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请问各位大神，需要做人巨噬细胞的F4/80,CD11b，CD163标记物，用流式，抗体哪家比较好呢，请推荐，谢谢！
比如，你使用了小鼠IgG1, PE 标记的特异性抗体，那就选用小鼠IgG1， PE标记的非特异性抗体。
下面这个图，是一个比较典型的使用了同型对照的结果图向左转|向右转灰色的曲线是未染色的样本，黄色是同型对照，红色是特异性抗体染色。由于细胞对抗体的非特异性结合，一般同型对照都会比未染色样本的荧光信号要强。所以设置阴性阈值的时候，要根据同型对照，而不是未染色的细胞。
请问一下做流式需要的抗体那种比较好，有人说Biolegend，eBioscience，santa，BD。求各位大神指教
如果一个抗体可以用来“做western blot，免疫荧光，免疫共沉淀,ELISA”，我估计着是HRP或碱性磷酸酶或生物素之类标记的，然后需要再加荧光标记的二抗，这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光（直接染色），效果还不错，但是反过来就没试过。
楼上两位还是要仔细看下产品说明书
