
PrimeSTAR GXL Premix is a convenient 2X PCR master mix of Takara"s PrimeSTAR GXL DNA polymerase. PrimeSTAR GXL is a hot-start, high-fidelity enzyme that is optimized to amplify GC-rich templates, samples with excess templates, and long PCR products up to 30 kb.
PrimeSTAR GXL Premix is a convenient 2X PCR master mix of Takara"s PrimeSTAR GXL DNA polymerase. PrimeSTAR GXL is a hot-start, high-fidelity enzyme that is optimized to amplify GC-rich templates, samples with excess templates, and long PCR products up to 30 kb.
PrimeSTAR GXL Premix enables you to simplify your amplification workflow, saving you time and reducing potential errors. Simply add primers and template, and you are ready to go.
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如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
利用CD3/CD4/IL-17的抗体组合(不同荧光素标记),具体步骤可按照淋巴细胞TH1/2亚型分析的protocol!
一般都是先做CD4的surface staining
然后再做IFN-gamma和IL-17的intrecellular staining
测流式后不用水洗
不用问为什么?_?
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