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TRAF2 antibody
Catalog Number: orb97673
| Product Name | TRAF2 antibody |
|---|---|
| Catalog Number | orb97673 |
| Species/Host | Mouse |
| Reactivity | Human |
| Conjugation | Unconjugated |
| Tested applications | ELISA, ICC, IF, WB |
| Immunogen | This TRAF2 monoclonal antibody is generated from mouse immunized with TRAF2 recombinant protein. |
| Target | TRAF2. This TRAF2 monoclonal antibody is generated from mouse immunized with TRAF2 recombinant protein. |
| Alternative Names | (click to expand) |
| Form/Appearance | PBS with 0.09% (W/V) sodium azide |
|---|---|
| Storage | Store at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Purity | This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| MW | 55.859 kDa |
| Clone ID | 214CT16.3.4 |
| Uniprot ID | Q12933 (Human) |
| NCBI | NP_066961.2 |
| Entrez | 7186 |
Mouse monoclonal to TRAF2.This TRAF2 monoclonal antibody is generated from mouse immunized with TRAF2 recombinant protein.
| Dilution Range | WB: 1:100-1000, IF/ICC: 1:10-50 |
|---|


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如题,试剂商推荐这家的,然后网上查了一下就是武汉三鹰啊,国产的,有点虚啊?请前辈指点迷津啊
尽量使用已经用荧光素标记好的单克隆抗体。
如果是单色实验,荧光素的亮度越强越好,比如标记了APC的抗体就要比标记了pacific blue的在相同抗原量,抗体用量相同的情况下,要亮很多
如果是多色实验,除了不要使用光谱重叠度高的荧光素以外,还要搭配染色指数。简单的说,就是用亮度强的荧光素标记的抗体来染水平低的抗原,而用较弱的荧光素标记的抗体来染高表达的抗原。
高保真生物技术有限公司~~实验整体外包服务商~~~
anti-mouse TCR γ/δ Antibody
anti-human TCR γ/δ Antibody
利用CD3/CD4/IL-17的抗体组合(不同荧光素标记),具体步骤可按照淋巴细胞TH1/2亚型分析的protocol!
一般都是先做CD4的surface staining
然后再做IFN-gamma和IL-17的intrecellular staining

