
- Synonym
- Source
- Molecular Characterization
This 200x detergent solution contains 10%(w/v) DDM and 2% (w/v) CHS that is necessary for solubilization of membrane proteins.DDM, or n-Dodecyl β-D-Maltoside [CAS: 69227-93-6], is a maltoside based non-ionic detergent with a hydrophilic maltose head and a hydrophobic long chain alkyl tail. CHS, or Cholesteryl Hemisuccinate Tris Salt [CAS: 102601-49-0], is an ionizable anionic detergent that stabilizes large unilamellar vesicles. DDM/CHS micelles provide a more membrane-like environment for solubilization of membrane proteins, and help to keep structural integrity and activity. This 200x detergent solution is sterile-filtered and contains no other preservatives.
- ApplicationThe 200x DDM/CHS solution is INDISPENSABLE to maintain solubilization and activity of membrane proteins (e.g. Full length CD20 protein, CD0-H52H3, CD0-H82E5, and CD133 protein CD3-H55H7), and should be used together with membrane proteins to dilute membrane proteins or antibodies, to prepare running buffer in SPR or BLI assay, etc.The 200x DDM/CHS solution should be diluted with assay buffer solutions to prepare 1x working solution just before experiment.
- Endotoxin
- Formulation

200x DDM CHS buffer on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than .
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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比如,你使用了小鼠IgG1, PE 标记的特异性抗体,那就选用小鼠IgG1, PE标记的非特异性抗体。
下面这个图,是一个比较典型的使用了同型对照的结果图向左转|向右转灰色的曲线是未染色的样本,黄色是同型对照,红色是特异性抗体染色。由于细胞对抗体的非特异性结合,一般同型对照都会比未染色样本的荧光信号要强。所以设置阴性阈值的时候,要根据同型对照,而不是未染色的细胞。
对于日常的肿瘤细胞株而言,大多数两种培养液都是可以的,但是有一点,就是在同一个细胞株中间不要频繁的切换两种培养液,因为毕竟两种培养液的成分有不同,在切换的过程中细胞有一个适应的过程,而这个过程中细胞的状态就不是很好,会出现黑色颗粒的物质。
流式细胞仪能检测多少个通道
需要同时检测检测多少个指标
厂家的抗体有多少种荧光标记
如果流式细胞仪的通道越多,那么同一份样本能同时做的表面/胞内标志就越多
A、 如何根据流式细胞仪搭配流式抗体
1) BD流式细胞仪
BD流式试剂选择及应用的相关问答与简介
流式细胞仪能检测多少个通道是由有多少根激光决定的以及每根激光的功能决定的,所以首先需要注意两点:
流式细胞仪有哪些激光。比如标配的FACSCalibur只有一根488nm的激光,能做FL1、FL2、FL3三个荧光通道/三个颜色,而选配的Calibur (FACSCalibur的简称)配有488nm和635nm两根激光,可以检测FL1-FL4四个通道/4个颜色。
不同型号的流式细胞仪的同样的一个通道检测荧光素的能力是不一样的,比如Calibur的FL3(第3通道)能检测PE-Texas Red, PE-Cy5, PerCP, PerCPCy5.5,PE-Cy7,而Aria的第3通道只能检测PE-Texas Red, PE-Cy5, PerCP。 Calibur第3通道能检测的PerCP-Cy5.5在FACSAria上是第4通道检测。Calibur和LSR都能检测4个颜色,但是第4个通道检测荧光素是不一样的

