Polyclonal Antibody to Epidermal Growth Factor (EGF)
URG; Beta-Urogastrone; Pro-epidermal growth factor
- Product No.PAA560Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig TypeIgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- ImmunogenRPA560Ra01-Recombinant Epidermal Growth Factor (EGF)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration0.77mg/ml
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IP.If the antibody is used in flow cytometry, please check FCM antibodies.
- DownloadInstruction Manual
- UOM20µl100µl200µl1ml10ml
- FOBUS$ 80 For more details, please contact local distributors!US$ 186 For more details, please contact local distributors!US$ 266 For more details, please contact local distributors!US$ 665 For more details, please contact local distributors!US$ 2660 For more details, please contact local distributors!
SPECIFITY of the Polyclonal Antibody to Epidermal Growth Factor (EGF)
The antibody isa rabbit polyclonal antibody raised against EGF. It has been selected for its ability to recognize EGF in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Epidermal Growth Factor (EGF)
Western blotting: 0.5-2µg/mL;1:380-1500Immunohistochemistry: 5-20µg/mL;1:38-150Immunocytochemistry: 5-20µg/mL;1:38-150Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Epidermal Growth Factor (EGF)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Epidermal Growth Factor (EGF)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized ServiceProtein A/G Purification ColumnStaining Solution for Cells and TissuePositive Controlfor AntibodyTissue/Sections Customized ServicePhosphorylated Antibody Customized ServiceWestern Blot (WB) Experiment ServiceImmunohistochemistry (IHC) Experiment ServiceImmunocytochemistry (ICC) Experiment ServiceFlow Cytometry (FCM) Experiment ServiceImmunoprecipitation (IP) Experiment ServiceImmunofluorescence (IF) Experiment ServiceBufferDAB Chromogen KitSABC KitLong-arm Biotin Labeling KitReal Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Rattus norvegicus (Rat) | Applications (RESEARCH USE ONLY!) |
| APA560Ra01 | Active Epidermal Growth Factor (EGF) | Cell culture; Activity Assays. |
| RPA560Ra01 | Recombinant Epidermal Growth Factor (EGF) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA560Ra01 | Polyclonal Antibody to Epidermal Growth Factor (EGF) | WB; IHC; ICC; IP. |
| MAA560Ra21 | Monoclonal Antibody to Epidermal Growth Factor (EGF) | WB; IHC; ICC; IP. |
| LAA560Ra72 | Biotin-Linked Monoclonal Antibody to Epidermal Growth Factor (EGF) | WB; IHC; ICC. |
| SEA560Ra | ELISA Kit for Epidermal Growth Factor (EGF) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA560Ra | CLIA Kit for Epidermal Growth Factor (EGF) | Chemiluminescent immunoassay for Antigen Detection. |
| PSA560Ra01 | Antibody Pair for Epidermal Growth Factor (EGF) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA560Ra01 | ELISA Kit DIY Materials for Epidermal Growth Factor (EGF) | Main materials for "Do It (ELISA Kit) Yourself". |
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
如题,试剂商推荐这家的,然后网上查了一下就是武汉三鹰啊,国产的,有点虚啊?请前辈指点迷津啊
尽量使用已经用荧光素标记好的单克隆抗体。
如果是单色实验,荧光素的亮度越强越好,比如标记了APC的抗体就要比标记了pacific blue的在相同抗原量,抗体用量相同的情况下,要亮很多
如果是多色实验,除了不要使用光谱重叠度高的荧光素以外,还要搭配染色指数。简单的说,就是用亮度强的荧光素标记的抗体来染水平低的抗原,而用较弱的荧光素标记的抗体来染高表达的抗原。
高保真生物技术有限公司~~实验整体外包服务商~~~
anti-mouse TCR γ/δ Antibody
anti-human TCR γ/δ Antibody
利用CD3/CD4/IL-17的抗体组合(不同荧光素标记),具体步骤可按照淋巴细胞TH1/2亚型分析的protocol!
一般都是先做CD4的surface staining
然后再做IFN-gamma和IL-17的intrecellular staining


