Anti-Vascular Endothelial Growth Factor A (VEGFA) Polyclonal Antibody
VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor
- Product No.FAA143Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Potency (Clone Number)n/a
- Ig TypeIgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Excitation LaserNone
- Vol. per test1-5 µl
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Organism Species Moren/a
- ApplicationsFlow cytometry.
- Downloadn/a
- UOM25test50test100test200test500test
- FOBUS$ 120 For more details, please contact local distributors!US$ 200 For more details, please contact local distributors!US$ 320 For more details, please contact local distributors!US$ 400 For more details, please contact local distributors!US$ 700 For more details, please contact local distributors!
USAGE of the Anti-Vascular Endothelial Growth Factor A (VEGFA) Polyclonal Antibody
For flow cytometry, the suggested use of this reagent is 1-5 µl per 10^6 cells in 100 µl volume. Optimal working dilutions could be determined by end user.
STORAGE of the Anti-Vascular Endothelial Growth Factor A (VEGFA) Polyclonal Antibody
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
GIVEAWAYS
INCREMENT SERVICES
Protein A/G Purification ColumnPositive Controlfor AntibodyTissue/Sections Customized ServicePhosphorylated Antibody Customized ServiceFlow Cytometry (FCM) Experiment ServiceImmunoprecipitation (IP) Experiment ServiceImmunofluorescence (IF) Experiment ServiceBufferDAB Chromogen KitSABC KitIsotype ControlReal Time PCR Experimental Service
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Catalog No. | Organism species: Rattus norvegicus (Rat) | Applications (RESEARCH USE ONLY!) |
RPA143Ra01 | Recombinant Vascular Endothelial Growth Factor A (VEGFA) | Positive Control; Immunogen; SDS-PAGE; WB. |
APA143Ra61 | Active Vascular Endothelial Growth Factor A (VEGFA) | Cell culture; Activity Assays. |
EPA143Ra61 | Eukaryotic Vascular Endothelial Growth Factor A (VEGFA) | Positive Control; Immunogen; SDS-PAGE; WB. |
APA143Ra01 | Active Vascular Endothelial Growth Factor A (VEGFA) | Cell culture; Activity Assays. |
RPA143Ra02 | Recombinant Vascular Endothelial Growth Factor A (VEGFA) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA143Ra02 | Polyclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA) | WB; IHC; ICC; IP. |
PAA143Ra01 | Polyclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA) | WB; IHC; ICC; IP. |
FAA143Ra01 | Anti-Vascular Endothelial Growth Factor A (VEGFA) Polyclonal Antibody | Flow cytometry. |
LAA143Ra81 | FITC-Linked Polyclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA) | WB; IHC; ICC; IF. |
LAA143Ra71 | Biotin-Linked Polyclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA) | WB; IHC; ICC. |
MAA143Ra21 | Monoclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA) | WB; IHC; ICC; IP. |
MAA143Ra22 | Monoclonal Antibody to Vascular Endothelial Growth Factor A (VEGFA) | WB; IHC; ICC; IP. |
SEA143Ra | ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA143Ra | CLIA Kit for Vascular Endothelial Growth Factor A (VEGFA) | Chemiluminescent immunoassay for Antigen Detection. |
KSA143Ra01 | ELISA Kit DIY Materials for Vascular Endothelial Growth Factor A (VEGFA) | Main materials for "Do It (ELISA Kit) Yourself". |
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血清内含有抗体,封闭的时候,选用同物种的血清,比如,使用小鼠单抗的话,就选用小鼠血清来进行封闭。这样,如果细胞表面有可以非特异性结合小鼠抗体的部位都会事先被结合,就不会再结合荧光标记的特异性抗体了。
如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
比如,你使用了小鼠IgG1, PE 标记的特异性抗体,那就选用小鼠IgG1, PE标记的非特异性抗体。
下面这个图,是一个比较典型的使用了同型对照的结果图向左转|向右转灰色的曲线是未染色的样本,黄色是同型对照,红色是特异性抗体染色。由于细胞对抗体的非特异性结合,一般同型对照都会比未染色样本的荧光信号要强。所以设置阴性阈值的时候,要根据同型对照,而不是未染色的细胞。