- Overview
- Data/Specifications
- Literature/Support
- How To Use
- Related Products
Overview
Type I collagen is the most abundant collagen and is found in connective tissues including tendon, ligament, dermis and blood vessel. It is the major component and the primary determinant of tensile strength of the extracelluar matrix (ECM). It is widely used as a thin layer on tissue-culture surfaces to enhance the attachment and proliferation of a variety of cells including endothelial cells, fibroblasts, hepatocytes, epithelial cells etc. In addition, collagen I can self-assemble into a 3-D superamolecular gel in vitro, making it an ideal biological scaffold to promote more in vivo-like cellular morphology and function.
Symbols/Related Terms:
- COL1A1
- COL1A2
- osteogenesis imperfecta
Data/Specifications
Reconstitution: Use 0.5 M acetic acid, pH 2.5 at 4°C. Collagen solubility 10 mg/ml. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils.
Purification: Partial pepsin digestion in acidic conditions and differential salt precipitation.
Source: Goat tissue. Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type I was purified by using differential salt precipitation.
Form: lyophilized, 10 mg/vial
Purity: SDS-PAGE 90%
Storage: Collagen dissolved in acetic acid is stable at 4 °C for 1 month. Lyophilized collagen long term storage (2 years) at -20°C or lower.
Literature/Support
Goat Type I Collagen Insert (PDF)
Note: inserts are for review only. Please use the insert that was sent with the product to ensure that the correct revision insert is being used for the product purchased.
How To Use
Key Applicatons:
- Used for goat collagen type I standard.
- Antigen for antibody production.
- Coating material for cell culture studies.
- Collagen microfibrils will activate platelets causing aggregation.
Note: may not be appropriate for 3D Gel formation
Related Products
ebiomall.com
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如题，试剂商推荐这家的，然后网上查了一下就是武汉三鹰啊，国产的，有点虚啊？请前辈指点迷津啊
尽量使用已经用荧光素标记好的单克隆抗体。
如果是单色实验，荧光素的亮度越强越好，比如标记了APC的抗体就要比标记了pacific blue的在相同抗原量，抗体用量相同的情况下，要亮很多
如果是多色实验，除了不要使用光谱重叠度高的荧光素以外，还要搭配染色指数。简单的说，就是用亮度强的荧光素标记的抗体来染水平低的抗原，而用较弱的荧光素标记的抗体来染高表达的抗原。
高保真生物技术有限公司~~实验整体外包服务商~~~
anti-mouse TCR γ/δ Antibody
anti-human TCR γ/δ Antibody
利用CD3/CD4/IL-17的抗体组合（不同荧光素标记），具体步骤可按照淋巴细胞TH1/2亚型分析的protocol！
一般都是先做CD4的surface staining
然后再做IFN-gamma和IL-17的intrecellular staining
