
We offer a variety of accessories, plasticware, and other consumables for the ICELL8 cx Single-Cell System: plates, chips, chip loading and collection kits, etc. Use these consumables together with our validated ICELL8 cx workflows or your own custom workflows to generate NGS libraries for your single-cell applications.
We offer a variety of accessories, plasticware, and other consumables for the ICELL8 cx Single-Cell System: plates, chips, chip loading & collection kits, etc. Use these consumables together with our validated ICELL8 cx workflows or your own custom workflows to generate NGS libraries for your single-cell applications.
As part of the open design of the platform, we also offer a variety of blank chips containing no preprinted oligos for the development of custom applications. Blank chips can also be used to test cell dispensing and imaging using the ICELL8 cx system. Two depth options are available to accommodate different reaction volumes. In the chip name, the number denotes the volume of the nanowells while the letter(s) designation following the number indicates the shape of the well. (See table titled "Formats of available ICELL8 cx blank chips" under More Information.)
- ICELL8 cx Blank Chip - 250 nl FB
- ICELL8 350v Chip
The following products are required for processing ICELL8 cx chips:
- The ICELL8 cx Loading Kit contains films and blotting paper designed to be used in the processing of ICELL8 cx chips
- The ICELL8 Loading Kit – B contains blotting paper, optical film, and sealing film to be used for processing ICELL8 chips in workflows with multiple incubation steps
- The ICELL8 Collection Kit – L contains a collection fixture to hold the chip, 2.0-ml tubes, and a film to allow users to spin down and collect the contents of a processed ICELL8 cx chip (e.g., extracted cDNA) into a tube for experiments
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尽量使用已经用荧光素标记好的单克隆抗体。
如果是单色实验,荧光素的亮度越强越好,比如标记了APC的抗体就要比标记了pacific blue的在相同抗原量,抗体用量相同的情况下,要亮很多
如果是多色实验,除了不要使用光谱重叠度高的荧光素以外,还要搭配染色指数。简单的说,就是用亮度强的荧光素标记的抗体来染水平低的抗原,而用较弱的荧光素标记的抗体来染高表达的抗原。
英 [ˈfɔ:mæt]美 [ˈfɔ:rmæt]
n. (出版物的)版式;[自](数据安排的)形式;电视节目的总安排(或计划)
vt. 使格式化;安排…的格局;设计…的版面
vi. 设计一个版式
如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
楼上两位还是要仔细看下产品说明书

