
Takara-brand Premix Ex Taq is an optimized 2X PCR master mix composed of Takara Ex Taq polymerase blend, Ex Taq reaction buffer (Mg2+), and dNTP mixture. Premix Ex Taq is designed for quick and easy assembly (via dilution only) of the PCR master mix.
Takara-brand Premix Ex Taq is an optimized 2X PCR master mix composed of Takara Ex Taq polymerase blend, Ex Taq reaction buffer (Mg2+), and dNTP mixture. Premix Ex Taq is designed for quick and easy assembly (via dilution only) of the PCR master mix.
A loading dye-added 2X master mix (PerfectShotEx Taq DNA Polymerase) is available for convenient loading of PCR products in agarose gels.
ExTaqis also available in the following formats:
- Individual components—including Ex Taq polymerase mix, dNTP mixture, and 10X reaction buffer (with or without Mg2+) for individual reaction setup and optimization.
- An antibody-mediated hot-start version—for preventing non-specific amplification from mispriming during reaction setup.
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请问各位大神,需要做人巨噬细胞的F4/80,CD11b,CD163标记物,用流式,抗体哪家比较好呢,请推荐,谢谢!
比如,你使用了小鼠IgG1, PE 标记的特异性抗体,那就选用小鼠IgG1, PE标记的非特异性抗体。
下面这个图,是一个比较典型的使用了同型对照的结果图向左转|向右转灰色的曲线是未染色的样本,黄色是同型对照,红色是特异性抗体染色。由于细胞对抗体的非特异性结合,一般同型对照都会比未染色样本的荧光信号要强。所以设置阴性阈值的时候,要根据同型对照,而不是未染色的细胞。
请问一下做流式需要的抗体那种比较好,有人说Biolegend,eBioscience,santa,BD。求各位大神指教
如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
楼上两位还是要仔细看下产品说明书

