
Antibiotics for the selection and maintenance of eukaryotic cells that are stably transfected with vectors expressing neomycin, puromycin, and hygromycin resistance cassettes.
Antibiotics for the selection and maintenance of eukaryotic cells that are stably transfected with vectors expressing neomycin, puromycin, and hygromycin resistance cassettes.
G418, Puromycin, & Hygromycin B dose concentrations for selection & maintenance
Antibiotic | |||
G418 | |||
Puromycin | |||
Hygromycin B |
For selecting colonies, the appropriate dose must be determined empirically for each specific cell line. We recommend testing a dosage range using dishes of untransfected cells and choosing the dose that kills all of the cells in 3–5 days. If all of the cells die in less than 24 hours, you should use a lower dose.
ebiomall.com






>
>
>
>
>
>
>
>
>
>
>
如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
楼上两位还是要仔细看下产品说明书
请问一下做流式需要的抗体那种比较好,有人说Biolegend,eBioscience,santa,BD。求各位大神指教
有哪位比较明白的能给我提点建议么,这两天真是晕到家了。谢谢!

