
Product Name | Oxonol VI[Bis - (3 - propyl - 5 - oxoisoxazol - 4 - yl)pentamethine oxonol] |
Size | 100 mg |
Catalog # | AS-84704 |
US$ | $164 |
Oxonol VI is used as an optical indicator for membrane potentials in lipid vesicles. Oxonol VI is a sensitive slow-response membrane potential probe whose magnitude of optical response is much larger than that of faster response probes (Oxonol V). The fluorescence of Oxonol VI decreases upon membrane hyperpolarization. In general, slow response probes are suitable for detecting changes in average membrane potentials of nonexcitable cells caused by respiratory activity, ion-channel permeability, drug binding and other factors. | |
Detailed Information | ![]() ![]() |
Storage | -20°C desiccated and protected from light |
References | 1. Cheng Z, et al. (2004).Am J Physiol Regul Integr Comp Physiol 286, R625-33.2. Koo YE, et al. (2004).Anal Chem 76, 2498-505. 3. Wu CC, et al. (2004).Cereb Cortex 14, 543-54.4. Suksaweang S, et al. (2004).Dev Biol 266, 109-22. 5. Iriyama A, et al. (2004). Invest Ophthalmol Vis Sci 45, 943-7. 6. Vest RS, et al. (2004).Biophys J 86, 2251-60.7. Zhang X and Kirsch LE (2004).Pharm Dev Technol 9, 219-27. 8. Hefnawy MA and Abou-Zeid AM (2003).Acta Microbiol Pol 52, 53-64.9. Jordan MB, et al. (2003).Blood 101, 594-601.10. Bianchi L, et al. (2003).J Biol Chem 278, 12415-24.11. deAzevedo LC, et al. (2003).J Neurobiol 55, 288-98.12. Ramani K and Balasubramanian SV (2003).Biochim Biophys Acta 1618, 67-78. |
Molecular Weight | 316.352 |
Molecular Formula | C17H20N2O4 |
Spectral Properties | Abs/Em = 599/634 nm |
Solvent System | DMSO |
Product Citations | Mennerick, S. et al. (2010). Diverse Voltage-Sensitive Dyes Modulate GABAAReceptor Function. J Neurosci 30, 2871. |
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如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
楼上两位还是要仔细看下产品说明书
请问一下做流式需要的抗体那种比较好,有人说Biolegend,eBioscience,santa,BD。求各位大神指教
有哪位比较明白的能给我提点建议么,这两天真是晕到家了。谢谢!

