- SpeciesReactivityHuman
- SpecificityDetects humanIL-9indirectELISAsandWesternblots. IndirectELISAs, lessthan15%cross-reactivitywithrecombinantmouseIL-9andrecombinantratIL-9isobserved.
- SourcePolyclonalGoatIgG
- PurificationAntigenAffinity-purified
- ImmunogenS.frugiperdainsectovariancelllineSf21-derivedrecombinanthumanIL-9
Gln19-Ile144
Accession#P15248 - FormulationLyophilizedfroma0.2μmfilteredsolutioninPBSwithTrehalose.*Smallpacksize(SP)issuppliedasa0.2µmfilteredsolutioninPBS.
- EndotoxinLevel<0.10 eu="" per="" 1="" μg="" of="" the="" antibody="" by="" the="" lal="">0.10>
- LabelUnconjugated
- WesternBlot0.1µg/mLRecombinantHumanIL‑9(Catalog#209-IL)
- NeutralizationMeasuredbyitsABIlitytoneutralizeIL‑9-inducedproliferationintheMO7ehumanmegakaryocyticleukemiccellline.Avanzi,G.etal.(1988)Br.J.Haematol.69:359.TheNeutralizationDose(ND50)istypically2-5 µg/mLinthepresenceof5 ng/mLRecombinantHumanIL‑9.
- ReconstitutionReconstituteat0.2mg/mLinsterilePBS.
- ShippingTheproductisshippedatambienttemperature.Uponreceipt,storeitimmediatelyatthetemperaturerecommendedbelow.*Smallpacksize(SP)isshippedwithpolarpacks.Uponreceipt,storeitimmediatelyat-20to-70°C
- Stability&StorageUseamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
- 12monthsfromdateofreceipt,-20to-70°Cassupplied.
- 1month,2to8°Cundersterileconditionsafterreconstitution.
- 6months,-20to-70°Cundersterileconditionsafterreconstitution.
- LongName:Interleukin9
- EntrezGeneIDs:3578(Human);16198(Mouse)
- AlternateNames:CytokineP40;HP40;IL9;IL-9;IL-9homologofmouseTcellandmastcellgrowthfactor40;interleukin9;interleukin-9;p40cytokine;p40T-cellandmastcellgrowthfactor;P40;T-cellgrowthfactorP40
Background:
HumanIL-9wasoriginallyidentifiedasacytokinefoundintheconditionedmediumofahumanTcellleukemiavirustypeI(HTLV-I)transformedTcelllinethatismitogenicforthefactor-dependenthumanmegakaryoblasticleukemiccellline,M07e.TheCDNAencodingthiscytokinewassubsequentlyisolatedbyfunctionalexpressioncloningandfoundtobesimilartothemouseTcellgrowthfactorIII/P40.ThishumancytokineanditsmurinehomologuearenowdesignatedashumanandmouseIL-9.BesidesHTLV-Ior-IItransformedTcelllines,rhIL-9isalsoproducedbyactivatedhumanPBLs. HumanIL-9wasalsoreportedtobeexpressedbyprimaryandculturedHodgkinandReed-Sternberg(H-RS)cellsderivedfromHodgkin’sdiseasepatients,suggestingapossIBLeroleforrhIL-9inthedevelopmentofthepathophysiologyofHodgkin’sdisease.
HumanandmurineIL-9arealsocapableofenhancingin vitrosurvivalofhumanTcelllinesaswellassynergizingwithEpotosupporterythroidcolonyformationin vitro.However,themastcellenhancingactivityassociatedwithrmIL-9hasnotyetbeendemonstratedinthehumansystemandnohumanIL-9-dependentTcellcloneshavebeenidentified.
ThegeneforrhIL-9hasbeenmappedtohumanchromosome5.Asinthemousesystem,thehumanIL-9cDNAencodesa144aminoacidresidueprecursorproteinwithan18aminoacidsignalpeptidethatiscleavedtoformthematurecysteine-richproteinwithapredictedmolecularmassof14kDa.HumanIL-9containsfourpotentialN-linkedglycosylationsitesandthenativerhIL-9isahighlyglycosylatedprotein.HumanandmouseIL-9share56%and67%homologyattheaminoacidandnucleotidelevels,respectively.AlthoughmurineIL-9isactiveonhumancells,humanIL-9isnotactiveonmousecells.
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请问一下做流式需要的抗体那种比较好,有人说Biolegend,eBioscience,santa,BD。求各位大神指教
如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
楼上两位还是要仔细看下产品说明书
多抗虽然可能可以产生更强的信号,但是特异性差,染色后荧光强度和抗原水平不是线性关系,不同批次差异很大。所以流式检测不使用多抗。

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