Hepatocytes derived from human induced pluripotent stem (hiPS) cells using the Cellartis iPS Cell to Hepatocyte Differentiation System are an alternative to primary hepatocytes, as they exhibit sufficient expression levels of drug-metabolizing enzymes and transporters and demonstrate stable functionality over time in culture. In addition, hiPS cell-derived hepatocytes can provide an accurate reflection of the metabolic diversity observed in the human population.
Hepatocytes derived from human induced pluripotent stem (hiPS) cells using the Cellartis iPS Cell to Hepatocyte Differentiation System are an alternative to primary hepatocytes, as they exhibit sufficient expression levels of drug-metabolizing enzymes and transporters and demonstrate stable functionality over time in culture. In addition, hiPS cell-derived hepatocytes can provide an accurate reflection of the metabolic diversity observed in the human population. The Cellartis iPS Cell to Hepatocyte Differentiation System provides a complete solution for generating functional, hiPS cell-derived hepatocytes within three weeks.
The process of generating hiPS cell-derived hepatocytes begins with the directed differentiation of hiPS cells into Definitive Endoderm (DE) cells that are then differentiated further into hepatocytes. The complete system provides media and ready-to-use coatings for each step of the iPS-cell-to-hepatocyte differentiation protocol. Starting with approximately 3 x 106 undifferentiated hiPS cells, this system yields 5 x 106 hepatocytes, equivalent to a confluent monolayer of 50 cm2. This do-it-yourself system offers a solution for the consistent production of assay-ready cells from patient-derived cells or from Cellartis brand iPS cell lines—enabling highly reproducible results.
Hepatocytes obtained with the Cellartis iPS Cell to Hepatocyte Differentiation System express major hepatic markers and show relevant CYP enzyme activities based on the genetic background of the original hiPS cells from which they were derived. In addition, these freshly differentiated hepatocytes are functionally stable for a longer timeframe compared to cryopreserved primary hepatocytes, enabling prolonged experiments such as long-term toxicity assays or viral infection studies. Cellartis Hepatocyte Maintenance Medium (Cat. # Y30051) is recommended for long-term maintenance of hiPS cell-derived hepatocytes.
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英 [ˈfɔ:mæt]美 [ˈfɔ:rmæt]
n. （出版物的）版式;[自]（数据安排的）形式;电视节目的总安排（或计划）
vt. 使格式化;安排…的格局;设计…的版面
vi. 设计一个版式
如果一个抗体可以用来“做western blot，免疫荧光，免疫共沉淀,ELISA”，我估计着是HRP或碱性磷酸酶或生物素之类标记的，然后需要再加荧光标记的二抗，这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光（直接染色），效果还不错，但是反过来就没试过。
流式细胞仪能检测多少个通道
需要同时检测检测多少个指标
厂家的抗体有多少种荧光标记
如果流式细胞仪的通道越多，那么同一份样本能同时做的表面/胞内标志就越多
A、 如何根据流式细胞仪搭配流式抗体
1） BD流式细胞仪
BD流式试剂选择及应用的相关问答与简介
流式细胞仪能检测多少个通道是由有多少根激光决定的以及每根激光的功能决定的，所以首先需要注意两点：
流式细胞仪有哪些激光。比如标配的FACSCalibur只有一根488nm的激光，能做FL1、FL2、FL3三个荧光通道/三个颜色，而选配的Calibur (FACSCalibur的简称)配有488nm和635nm两根激光，可以检测FL1-FL4四个通道/4个颜色。
不同型号的流式细胞仪的同样的一个通道检测荧光素的能力是不一样的，比如Calibur的FL3（第3通道）能检测PE-Texas Red, PE-Cy5, PerCP, PerCPCy5.5,PE-Cy7,而Aria的第3通道只能检测PE-Texas Red, PE-Cy5, PerCP。 Calibur第3通道能检测的PerCP-Cy5.5在FACSAria上是第4通道检测。Calibur和LSR都能检测4个颜色，但是第4个通道检测荧光素是不一样的
能用的条件：1.分子的小鼠和人差异小，2.小鼠细胞本身不含有鼠抗体的结合受体；
不能用的条件：1.分子种属间差异大，2.采用的小鼠细胞株表面有鼠抗体的结合受体。
推荐尽量采用抗鼠的抗体，义翘神州兔单抗技术做出来的FCM抗体不少，可以到官网搜一下看看。
