Our cell-cycle reporter vectors allow for real-time monitoring of cell-cycle progression in living cells without fixation. They even let you visualize cell shape.
Our cell-cycle reporter vectors allow for real-time monitoring of cell-cycle progression in living cells without fixation. They even let you visualize cell shape.
Fucci probes
Cell-cycle reporter vectors deliver fluorescent, ubiquitination-based cell-cycle indicators (Fucci) that allow you to identify cells in various phases of the cell cycle. These Fucci probes contain Cdt1 or Geminin, proteins whose levels fluctuate differentially throughout the cell cycle: Cdt1 levels peak in G1 phase; as cells transition into S phase, Cdt1 levels fall and Geminin levels rise, remaining high until the cells are back in G1. Cells control Cdt1 and Geminin levels post-translationally, using ubiquitination to target the unwanted proteins for proteasomal degradation. To allow Geminin and Cdt1 to be easily visualized, each is expressed with a red or cyan Living Colors fluorescent tag. These probes allow precise, visual evaluation of the cell-cycle phase.
Monitor cell cycle and cell shape in real time
Our Fucci cell-cycle reporters let you label just the nucleus, or both the nucleus and cytoplasm, allowing visualization of cell shape. Although most Fucci probes label only cell nuclei, we offer a truncated version of hGeminin that is able to migrate to the cytoplasm between S and M phases, enabling visualization of cell morphology.
A variety of Fucci probes and delivery options
We offer retrovirus and plasmid-based vectors for the delivery of a variety of Fucci probes. Each probe consists of a red or cyan fluorescent tag, and a deletion mutant of either hCdt1 or hGeminin. Our retroviral vectors can either be transfected as plasmids or transduced as retrovirus in a wide variety of mammalian cells. Once integrated into the host genome, these vectors provide stable, heritable expression of the probes.
The plasmid-based vector, pTRE-CellCycle, allows tightly controlled, tetracycline (Tet)-inducible expression of two Fucci probes from a bidirectional promoter that can be induced using any Tet-On or Tet-Off technology. Expression of these probes causes cell nuclei to turn red during G1 phase and cyan during S, G2, and M phases, allowing complete visual tracking of the cell cycle.
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如题，试剂商推荐这家的，然后网上查了一下就是武汉三鹰啊，国产的，有点虚啊？请前辈指点迷津啊
尽量使用已经用荧光素标记好的单克隆抗体。
如果是单色实验，荧光素的亮度越强越好，比如标记了APC的抗体就要比标记了pacific blue的在相同抗原量，抗体用量相同的情况下，要亮很多
如果是多色实验，除了不要使用光谱重叠度高的荧光素以外，还要搭配染色指数。简单的说，就是用亮度强的荧光素标记的抗体来染水平低的抗原，而用较弱的荧光素标记的抗体来染高表达的抗原。
高保真生物技术有限公司~~实验整体外包服务商~~~
anti-mouse TCR γ/δ Antibody
anti-human TCR γ/δ Antibody
利用CD3/CD4/IL-17的抗体组合（不同荧光素标记），具体步骤可按照淋巴细胞TH1/2亚型分析的protocol！
一般都是先做CD4的surface staining
然后再做IFN-gamma和IL-17的intrecellular staining
