![Iwai-Chem/Anti-Mouse CD3ε Monoclonal Antibody [145-2C11]/Azide Free & Low Endotoxin/E-AB-F1103-Azide Free & Low Endotoxin](images/Iwai-Chem/E-AB-F1103C-FC01__31236.1589584780.jpg)
Product Description
Anti-Mouse CD3ε Monoclonal Antibody
Product Details
Alternate Names | T-cell surface glycoprotein CD3 epsilon chain,CD3E,T-cell surface antigen T3/Leu-4 epsilon chain,CD3e,CD3E,T3E |
Clone No. | 145-2C11 |
Reactivity | Mouse |
Host | Armenian Hamster |
Isotype | Armenian Hamster IgG |
Uniprot ID | P22646 |
Background | CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. |
Datasheet & Specification (Click link)
Catalog No. | Product Name | Sizes |
E-AB-F11030 | AF/LE Purified Anti-Mouse CD3ε Antibody[145-2C11] | 50 μg,500 μg,1 mg |
E-AB-F1103A | Purified Anti-Mouse CD3ε Antibody[145-2C11] | 50 μg,100 μg |
E-AB-F1103B | Biotin Anti-Mouse CD3ε Antibody[145-2C11] | 50 μg,100 μg |
E-AB-F1103C | FITC Anti-Mouse CD3ε Antibody[145-2C11] | 50 Tests,100 Tests,500 Tests |
E-AB-F1103D | PE Anti-Mouse CD3ε Antibody[145-2C11] | 50 Tests,100 Tests,500 Tests |
E-AB-F1103E | APC Anti-Mouse CD3ε Antibody[145-2C11] | 50 Tests,100 Tests,500 Tests |
E-AB-F1103G | PE/Cyanine5 Anti-Mouse CD3ε Antibody[145-2C11] | 20 Tests,50 Tests,100 Tests,500 Tests |
E-AB-F1103H | PE/Cyanine7 Anti-Mouse CD3ε Antibody[145-2C11] | 20 Tests,50 Tests,100 Tests,500 Tests |
E-AB-F1103I | PE/Cyanine5.5 Anti-Mouse CD3ε Antibody[145-2C11] | 20 Tests,50 Tests,100 Tests,500 Tests |
E-AB-F1103J | PerCP/Cyanine5.5 Anti-Mouse CD3ε Antibody[145-2C11] | 20 Tests,50 Tests,100 Tests,500 Tests |
E-AB-F1103L | AF488 Anti-Mouse CD3ε Antibody[145-2C11] | 20 Tests,50 Tests,100 Tests,500 Tests |
E-AB-F1103M | AF647 Anti-Mouse CD3ε Antibody[145-2C11] | 20 Tests,50 Tests,100 Tests,500 Tests |
References
Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off .
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
* Remark Icon :
WB=Western Blotting, IP=Immunoprecipitation, IF=Immunofluorescence, IHC=Immunohistochemistory, IHC-p=Immunohistochemistory Paraffin, FCM=Flow Cytometry, CH=ChIP Assay
Manufactured by: Elabscience
Questions about this product? Ready to place an order? Email us at orders@iwai-chem.comor give us a call: (650) 486-1541 |
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说得简单点就是,细胞核是原封不动的,没有重组过,而细胞核外的东西是很多别的细胞中提取出来的并组合起来的。这样的混合体淋巴细胞产生的抗体具有很强的嵌合能力,针对性比一般的抗体强,但是它的来源没变,所以称之为混合单克隆抗体
羊多抗免疫步骤和兔多抗免疫步骤一样的,周期也是一样的,就是免疫的部位不同,羊的免疫部位是:羊的小腿内侧皮下。采血部位是:颈静脉;放血部位是:颈静脉;最后收集血的部位:颈动脉。希望对你有帮助。
抗体的特异性鉴定 抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在 IC50时的浓度,并按下列公式计算交叉反应率。 如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示 这一抗血清与其他抗原物质的交叉反应率近似为 0,即该血清的特异性较好。
抗体的亲和力 是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小、抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键、疏水键、侧链相反电荷基因的库仑力、范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol,通常K的范围在 108 ~1010 /mol,也有多达 1014 /mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。向左转|向右转
一般来说多克隆的阳性率高一些,但出现假阳性的比例也高一些。
没有marker,怎么知道你做的蛋白大小?
没有参照物,怎么知道你跑的快不快?
没有尺子,怎么知道你的size大小?
凭嘴说吗?
1取组织
(1) 灌注固定:暴露心脏,将注射针头插入左心室,剪开右心耳,快速灌注PBS,待右心耳处流出澄清液体,换4%多聚甲醛灌注到大鼠四肢强直为止,快速取脑和肾。
(2) 固定:将组织置于4%多聚甲醛中固定15小时
(3) 脱水:将组织放入30% 蔗糖溶液中直至下沉
(4) 切片:脑组织冰冻切片20微米,37度烘干2h以上;
(5)将切片放置-80度保存,随用随取。
2免疫单标记
(1)做抗原修复(0.4g柠檬酸、3g柠檬酸钠、1000ml水配制成溶液,放到水浴箱中90~94℃保存15min),冷却后, 用1*PBS反复洗标本10min,吸去PBS,同时向标本加渗透液(配0.4%trition液,然后将BSA加入到配好的trition液中,配成1%浓度)100ul,常温下湿盒反应1h。
(2) 吸去渗透液,同时向标本加block液(5%封闭用正常山羊血清)100ul,常温下湿盒反应5h。
(3) 吸去block液,同时向标本加入1:50稀释的羊抗鼠一抗100ul,37度湿盒反应过夜。
(4)吸去一抗,并回收,用1*PBS洗液反复洗15min。
(5)快速从冰箱中取出驴抗羊的二抗,并立即用锡纸包裹,注入到一个新的以锡纸包裹的tube管中并用PBS以1:200稀释。
(6)进入暗室,吸去PBS,同时加入二抗100ul,37度湿盒反应2h,封片。
(7)放置到荧光显微镜下观察。
多抗,稀释度更大,特异性相对较差,容易出现多条带。
兔的单克隆抗体和鼠的单克隆抗体在使用上不会有什么区别。
用来很多抗体,许多时候觉得单抗多抗也未必是理论上那样的。单抗做不好的也有,多抗条带唯一且清晰的也有。
而且很多蛋白的抗体未必有那么多的选择。

