![Iwai-Chem/Anti-Mouse CD3ε Monoclonal Antibody [145-2C11]/Azide Free & Low Endotoxin/E-AB-F1103-Azide Free & Low Endotoxin](images/Iwai-Chem/E-AB-F1103C-FC01__31236.1589584780.jpg)
Product Description
Anti-Mouse CD3ε Monoclonal Antibody
Product Details
Alternate Names | T-cell surface glycoprotein CD3 epsilon chain,CD3E,T-cell surface antigen T3/Leu-4 epsilon chain,CD3e,CD3E,T3E |
Clone No. | 145-2C11 |
Reactivity | Mouse |
Host | Armenian Hamster |
Isotype | Armenian Hamster IgG |
Uniprot ID | P22646 |
Background | CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. |
Datasheet & Specification (Click link)
Catalog No. | Product Name | Sizes |
E-AB-F11030 | AF/LE Purified Anti-Mouse CD3ε Antibody[145-2C11] | 50 μg,500 μg,1 mg |
E-AB-F1103A | Purified Anti-Mouse CD3ε Antibody[145-2C11] | 50 μg,100 μg |
E-AB-F1103B | Biotin Anti-Mouse CD3ε Antibody[145-2C11] | 50 μg,100 μg |
E-AB-F1103C | FITC Anti-Mouse CD3ε Antibody[145-2C11] | 50 Tests,100 Tests,500 Tests |
E-AB-F1103D | PE Anti-Mouse CD3ε Antibody[145-2C11] | 50 Tests,100 Tests,500 Tests |
E-AB-F1103E | APC Anti-Mouse CD3ε Antibody[145-2C11] | 50 Tests,100 Tests,500 Tests |
E-AB-F1103G | PE/Cyanine5 Anti-Mouse CD3ε Antibody[145-2C11] | 20 Tests,50 Tests,100 Tests,500 Tests |
E-AB-F1103H | PE/Cyanine7 Anti-Mouse CD3ε Antibody[145-2C11] | 20 Tests,50 Tests,100 Tests,500 Tests |
E-AB-F1103I | PE/Cyanine5.5 Anti-Mouse CD3ε Antibody[145-2C11] | 20 Tests,50 Tests,100 Tests,500 Tests |
E-AB-F1103J | PerCP/Cyanine5.5 Anti-Mouse CD3ε Antibody[145-2C11] | 20 Tests,50 Tests,100 Tests,500 Tests |
E-AB-F1103L | AF488 Anti-Mouse CD3ε Antibody[145-2C11] | 20 Tests,50 Tests,100 Tests,500 Tests |
E-AB-F1103M | AF647 Anti-Mouse CD3ε Antibody[145-2C11] | 20 Tests,50 Tests,100 Tests,500 Tests |
References
Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off .
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
* Remark Icon :
WB=Western Blotting, IP=Immunoprecipitation, IF=Immunofluorescence, IHC=Immunohistochemistory, IHC-p=Immunohistochemistory Paraffin, FCM=Flow Cytometry, CH=ChIP Assay
Manufactured by: Elabscience
Questions about this product? Ready to place an order? Email us at orders@iwai-chem.comor give us a call: (650) 486-1541 |
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又由于自然存在的抗原大都存在多个抗原表位,会刺激机体产生多种针对同一抗原的不同抗原表位相应的不同抗体.
制备抗体都需要进行纯化处理
一般来说多克隆的阳性率高一些,但出现假阳性的比例也高一些。
抗原有两个基本特性,即抗原性和免疫原性。有抗原性的物质不一定有免疫原性,所以由此引出半抗原和完全全抗原,半抗原必须经过经过一定的改造(偶联蛋白载体BSA,OVA或者HSA等大分子物质)方能成为完全。一般而言完全抗原分子量越大(大于10KDa),结构越复杂引起免疫反应的能力也就越强。
抗体就是能与特异性抗原结合的免疫球蛋白,抗体一般分为多克隆抗体和单克隆抗体,多克隆抗体能与抗原的多个表位结合。本篇主要讲述兔来源的多克隆抗体的生产步骤
多抗一般制备流程:完全抗原的准备→兔子的免疫→ 效价检测和终放→抗体亲和纯化→抗体的浓缩和保存。
1、制备抗原。
2、选择实验动物。
3、动物免疫。
4、试取血进行测试,看看是否成功免疫。
5、如果成功免疫,杀死实验动物,采集全部血清。
6、纯化出抗体。
7、鉴定抗体。包括纯度以及特异性。
二、抗体分类
抗原通常是由多个抗原决定簇组成的,由一种抗原决定簇刺激机体,由一个B淋巴细胞接受该抗原所产生的抗体称之为单克隆抗体(Monoclone antibody)。由多种抗原决定簇刺激机体,相应地就产生各种各样的单克隆抗体,这些单克隆抗体混杂在一起就是多克隆抗体,机体内所产生的抗体就是多克隆抗体;除了抗原决定簇的多样性以外,同样一类抗原决定簇,也可刺激机体产生IgG、IgM、IgA、IgE和IgD等五类抗体。多克隆抗体是由异源抗原(大分子抗原、半抗原偶联物)刺激机体产生免疫反应,有机体浆细胞分泌的一组免疫球蛋白。多克隆抗体由于其可识别多个抗原表位、可引起沉淀反应,制备时间短,成本低的原因广泛应用于研究和诊断方面。
三、免疫方法
可以采用以下各种方法之一进行免疫。
(1)淋巴结注射法:①在兔的两后足跖部皮下(或皮内)注射活卡介苗 50mg(每侧约0.30ml) 。7~10 天后,兔跖及腘肌淋巴结肿大;②于肿大的两侧淋巴结内各注射加有完全佐剂的IgG 乳化抗原 0.50ml(含 IgG 5mg/ml、青霉素 1 000U/ml、链霉素 1 000μg/ml);③必要时,14 天后,重复步骤②一次;④再过 7 天后,于两侧淋巴结内各注射加有完全佐剂的 IgG 乳化抗原0.50ml(含IgG5mg/ml、青霉素 1 000U/ml、链霉素 1 000μg/ml) ;⑤5~7天后,耳静脉采血。测定血清效价。
(2)皮下多点注射法:①家兔两侧掌(跖内各注射含有完全佐剂抗原 0.10ml(IgG 含量5mg/ml) ;②7~10 天后,脊柱两侧多点(颈、胸、腰椎各两点、共 6 点)皮下注射含不完全佐剂5的抗原,每点 0.50ml;③7~10 天后,脊柱两侧重复注射一次;④7~10 天后试血。不合格者重复步骤③。
(3)多途径联合注射法:①两侧掌(跖)内侧皮下注射含完全佐剂抗原 0.50ml(IgG 量为 5mg/ml) ;②14 天后,多点皮下注射含有不完全佐剂抗原;③7 天后,耳静脉注射不含佐剂的抗原 2ml;④测定血清抗体效价,不合格者重复步骤③,并适当递增 IgG 量。

