SKU#: 2101241001
Lot No. 03C01
Ig type: rabbit IgG
Size: 100μg/vial
Specificity: Human, rat, mouse. No cross reactivity with other proteins.
Immunogen: A synthetic peptide corresponding to a sequence near the C-terminal of human Annexin I, different from the related rat and mouse sequence by two amino acids.
Purity: Immunogen affinity purified.
Applications:
Western blot: At 1-2μg/ml with the appropriate system to detect Annexin I in cells and tissues.
Immunohistochemistry (P): At 1-2μg/ml to detect Annexin I in formalin fixed and paraffin embedded tissues. Digesting the sections is required.
Immunocytochemistry: Suitable
Other applications have not been tested.
Optimal dilutions should be determined by end user.
Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg, Thimerosal, 0.05mg NaN3.
Reconstitution: 0.2ml of distilled water will yield a concentration of 500μg/ml.
At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for longer time.
1. Wallner, B. P.; Mattaliano, R. J.; Hession, C.; Cate, R. L.; Tizard, R.; Sinclair, L. K.; Foeller, C.; Chow,
E. P.; Browning, J. L.; Ramachandran, K. L.; Pepinsky, R. B. : Cloning and expression of human
lipocortin, a phospholipase A2 inhibitor with potential anti-inflammatory activity. Nature 320: 77-81,
1986. .
2. Kovacic RT, Tizard R, Cate RL, FreyAZ, Wallner
BP. Correlation of gene and protein structure of rat and human lipocortin I. Biochemistry. 1991 Sep
17;30(37):9015-21.
3. Walther, A.; Riehemann, K.; Gerke, V. A novel ligand of the formyl peptide receptor: annexin I
regulates neutrophil extravasation by interacting with the FPR. Molec. Cell 5: 831-840, 2000.
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多抗,稀释度更大,特异性相对较差,容易出现多条带。
兔的单克隆抗体和鼠的单克隆抗体在使用上不会有什么区别。
用来很多抗体,许多时候觉得单抗多抗也未必是理论上那样的。单抗做不好的也有,多抗条带唯一且清晰的也有。
而且很多蛋白的抗体未必有那么多的选择。
其次,察看次目的蛋白的存在形式,有没有多聚体形式及变构形式;
最后,查看多家抗体公司的DATA,看看别人的WB做出来的条带的位置。
根据你说的,特异识别多个组织中的同样大小的条带,我觉得很可能就是你的目的蛋白。
单克隆抗体结构相似且均一,有高度特异性
又由于自然存在的抗原大都存在多个抗原表位,会刺激机体产生多种针对同一抗原的不同抗原表位相应的不同抗体.
抗原有两个基本特性,即抗原性和免疫原性。有抗原性的物质不一定有免疫原性,所以由此引出半抗原和完全全抗原,半抗原必须经过经过一定的改造(偶联蛋白载体BSA,OVA或者HSA等大分子物质)方能成为完全。一般而言完全抗原分子量越大(大于10KDa),结构越复杂引起免疫反应的能力也就越强。
抗体就是能与特异性抗原结合的免疫球蛋白,抗体一般分为多克隆抗体和单克隆抗体,多克隆抗体能与抗原的多个表位结合。本篇主要讲述兔来源的多克隆抗体的生产步骤
多抗一般制备流程:完全抗原的准备→兔子的免疫→ 效价检测和终放→抗体亲和纯化→抗体的浓缩和保存。