Type
PolyclonalAntibody
Applications
Westernblotting,Immunohistochemistry
AntibodiesApplications
SourceofAntigen
E.coli
Hosts
Sheep
Preparation
TheantibodywasraisedinsheepbyimmunizationwiththerecombinantHumanParaoxonase 1.
AminoAcidSequence
Theimmunizationantigen(40.68 kDa,calculated)isaproteincontaining355 AAofrecombinantHumanParaoxonase1. UniProtKBacc.no.P27169(Met1-Leu355).C-terminalHis-tag(7 extraAA).(highlighted).
MAKLIALTLLGMGLALFRNHQSSYQTRLNALREVQPVELPNCNLVKGIETGSEDLEILPNGLAFISSGLKYPGIKSFNPNSPGKILLMDLNEEDPTVLELGITGSKFDVSSFNPHGISTFTDEDNAMYLLVVNHPDAKSTVELFKFQEEEKSLLHLKTIRHKLLPNLNDIVAVGPEHFYGTNDHYFLDPYLQSWEMYLGLAWSYVVYYSPSEVRVVAEGFDFANGINISPDGKYVYIAELLAHKIHVYEKHANWTLTPLKSLDFNTLVDNISVDPETGDLWVGCHPNGMKIFFYDSENPPASEVLRIQNILTEEPKVTQVYAENGTVLQGSTVASVYKGKLLIGTVFHKALYCELEHHHHHH Human.Notyettestedinotherspecies. ImmunoaffinitychromatographyonacolumnwithimmobilizedrecombinantHumanParaoxonase 1. 0.1 mg(determinedbyBCAmethod,BSAwasusedasastandard) Theantibodyislyophilizedin0.05 Mphosphatebuffer,0.1 MNaCl,pH7.2. AZIDEFREE. Add0.2 mlofdeionizedwaterandletthelyophilizedpelletdissolvecompletely.Slightturbiditymayoccurafterreconstitution,whichdoesnotaffectactivityoftheantibody.Inthiscaseclarifythesolutionbycentrifugation. Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below. Thelyophilizedantibodyremainsstableandfullyactiveuntiltheexpirydatewhenstoredat –20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcyclesandstorefrozenat –80°C.Reconstitutedantibodycanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowdeclineinactivityafteroneweekat 4°C. IndirectELISA –todeterminetiteroftheantibody Thisproductisforresearchuseonly.SpeciesReactivity
PurificationMethod
AntibodyContent
Formulation
Reconstitution
Shipping
Storage/Expiration
QualityControlTest
SDSPAGE–todeterminepurityoftheantibodyNote