Type
PolyclonalAntibody
Applications
Westernblotting,ELISA,Immunohistochemistry
AntibodiesApplications
SourceofAntigen
E.coli
Hosts
Rabbit
Preparation
TheantibodywasraisedinrabbitsbyimmunizationwiththerecombinantHumanS100A16.
AminoAcidSequence
Total113 AA.MW:13,04 kDa(calculated).UniProtKBacc.no.Q96FQ6.N-TerminalHis-tag,10 extraAA(highlighted).
MKHHHHHHASMSDCYTELEKAVIVLVENFYKYVSKYSLVKNKISKSSFREMLQKELNHMLSDTGNRKAADKLIQNLDANHDGRISFDEYWTLIGGITGPIAKLIHEQEQQSSS
SpeciesReactivity
Human.Notyettestedinotherspecies.
PurificationMethod
ImmunoaffinitychromatographyonacolumnwithimmobilizedrecombinantHumanS100A16.
AntibodyContent
0.1 mg(determinedbyBCAmethod,BSAwasusedasastandard)
Formulation
Theantibodyislyophilizedin0.05 Mphosphatebuffer,0.1 MNaCl,pH7.2. AZIDEFREE.
Reconstitution
Add0.1 mlofdeionizedwaterandletthelyophilizedpelletdissolvecompletely.Slightturbiditymayoccurafterreconstitution,whichdoesnotaffectactivityoftheantibody.Inthiscaseclarifythesolutionbycentrifugation.
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Thelyophilizedantibodyremainsstableandfullyactiveuntiltheexpirydatewhenstoredat –20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcyclesandstorefrozenat –80°C.Reconstitutedantibodycanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowdeclineinactivityafteroneweekat 4°C.
QualityControlTest
IndirectELISA –todeterminetiteroftheantibody
SDSPAGE–todeterminepurityoftheantibody
Note
Thisproductisforresearchuseonly.