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基于腊梅花EST序列的抗性基因分析及几丁质酶基因克隆与表达...
来自 : mayitao
货号:E-AB-32152
抗体英文名:N/H/K-RasPolyclonalAntibody
应用范围:WB,IHC-p,ELISA
宿主:Rabbit
适应物种:Human,Mouse,Rat
克隆性:
保存条件:Storeat-20℃.Avoidfreeze/thawcycles.
亚型:IgG
免疫原:SynthesizedpeptidederivedfromtheN-terminalregionofhumanN/H/K-Ras
规格:30μL/60μL/120μL
Overview
SynonymsNRAS,HRAS1,GTPaseNRas,TransformingproteinN-Ras,HRAS,HRAS1,GTPaseHRas,H-Ras-1,Ha-Ras,Transformingproteinp21,c-H-ras,p21ras,KRAS,KRAS2,RASK2,GTPaseKRas,K-Ras2,Ki-Ras,c-K-ras,c-Ki-ras
SwissprotP01111/P01112/P01116
GeneID3265/3845/4893
SourceRabbit
ReactivityHuman,Mouse,Rat
ImmunogenSynthesizedpeptidederivedfromtheN-terminalregionofhumanN/H/K-Ras
ApplicationWB,IHC-p,ELISA
RecommendeddilutionWB1:500-1:2000,IHC1:100-1:300,ELISA1:20000
Concentration1mg/mL
ClonalityPolyclonal
ResearchAreasCancer,Cellbiology,Epigenetics
 
Properties
IsotypeIgG
PurificationAffinitypurification
ConjugationUnconjugated
CalculatedMV21kDa
ObservedMV21kDa
StorageinstructionsStoreat-20.Avoidfreeze/thawcycles.
StoragebufferPBSwith0.02%sodiumazide,0.5%BSAand50%glycerol,pH7.4
Background
 
NRASanN-rasoncogeneencodingamembraneproteinthatshuttlesbetweentheGolgiapparatusandtheplasmamembrane.ThisshuttlingisregulatedthroughpalmitoylationanddepalmitoylationbytheZDHHC9-GOLGA7complex.Theencodedprotein,whichhasintrinsicGTPaseactivity,isactivatedbyaguaninenucleotide-exchangefactorandinactivatedbyaGTPaseactivatingprotein.Mutationsinthisgenehavebeenassociatedwithsomaticrectalcancer,follicularthyroidcancer,autoimmunelymphoproliferativesyndrome,Noonansyndrome,andjuvenilemyelomonocyticleukemia.HRASbelongstotheRasoncogenefamily,whosemembersarerelatedtothetransforminggenesofmammaliansarcomaretroviruses.Theproductsencodedbythesegenesfunctioninsignaltransductionpathways.TheseproteinscanbindGTPandGDP,andtheyhaveintrinsicGTPaseactivity.KRAS,aKirstenrasoncogenehomologfromthemammalianrasgenefamily,encodesaproteinthatisamemberofthesmallGTPasesuperfamily.Asingleaminoacidsubstitutionisresponsibleforanactivatingmutation.Thetransformingproteinthatresultsisimplicatedinvariousmalignancies,includinglungadenocarcinoma,mucinousadenoma,ductalcarcinomaofthepancreasandcolorectalcarcinoma.

Image

WesternBlotanalysisof293TcellsusingN/H/K-RasPolyclonalAntibodyatdilutionof1:1000.

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