基于腊梅花EST序列的抗性基因分析及几丁质酶基因克隆与表达...
| 货号: | E-AB-32152 |
| 抗体英文名: | N/H/K-RasPolyclonalAntibody |
| 应用范围: | WB,IHC-p,ELISA |
| 宿主: | Rabbit |
| 适应物种: | Human,Mouse,Rat |
| 克隆性: | 否 |
| 保存条件: | Storeat-20℃.Avoidfreeze/thawcycles. |
| 亚型: | IgG |
| 免疫原: | SynthesizedpeptidederivedfromtheN-terminalregionofhumanN/H/K-Ras |
| 规格: | 30μL/60μL/120μL |
| Synonyms | NRAS,HRAS1,GTPaseNRas,TransformingproteinN-Ras,HRAS,HRAS1,GTPaseHRas,H-Ras-1,Ha-Ras,Transformingproteinp21,c-H-ras,p21ras,KRAS,KRAS2,RASK2,GTPaseKRas,K-Ras2,Ki-Ras,c-K-ras,c-Ki-ras |
| Swissprot | P01111/P01112/P01116 |
| GeneID | 3265/3845/4893 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | SynthesizedpeptidederivedfromtheN-terminalregionofhumanN/H/K-Ras |
| Application | WB,IHC-p,ELISA |
| Recommendeddilution | WB1:500-1:2000,IHC1:100-1:300,ELISA1:20000 |
| Concentration | 1mg/mL |
| Clonality | Polyclonal |
| ResearchAreas | Cancer,Cellbiology,Epigenetics |
Properties
| Isotype | IgG |
| Purification | Affinitypurification |
| Conjugation | Unconjugated |
| CalculatedMV | 21kDa |
| ObservedMV | 21kDa |
| Storageinstructions | Storeat-20℃.Avoidfreeze/thawcycles. |
| Storagebuffer | PBSwith0.02%sodiumazide,0.5%BSAand50%glycerol,pH7.4 |
| Background | NRASanN-rasoncogeneencodingamembraneproteinthatshuttlesbetweentheGolgiapparatusandtheplasmamembrane.ThisshuttlingisregulatedthroughpalmitoylationanddepalmitoylationbytheZDHHC9-GOLGA7complex.Theencodedprotein,whichhasintrinsicGTPaseactivity,isactivatedbyaguaninenucleotide-exchangefactorandinactivatedbyaGTPaseactivatingprotein.Mutationsinthisgenehavebeenassociatedwithsomaticrectalcancer,follicularthyroidcancer,autoimmunelymphoproliferativesyndrome,Noonansyndrome,andjuvenilemyelomonocyticleukemia.HRASbelongstotheRasoncogenefamily,whosemembersarerelatedtothetransforminggenesofmammaliansarcomaretroviruses.Theproductsencodedbythesegenesfunctioninsignaltransductionpathways.TheseproteinscanbindGTPandGDP,andtheyhaveintrinsicGTPaseactivity.KRAS,aKirstenrasoncogenehomologfromthemammalianrasgenefamily,encodesaproteinthatisamemberofthesmallGTPasesuperfamily.Asingleaminoacidsubstitutionisresponsibleforanactivatingmutation.Thetransformingproteinthatresultsisimplicatedinvariousmalignancies,includinglungadenocarcinoma,mucinousadenoma,ductalcarcinomaofthepancreasandcolorectalcarcinoma. |
Image

WesternBlotanalysisof293TcellsusingN/H/K-RasPolyclonalAntibodyatdilutionof1:1000.
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发布于 : 2017-07-24
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