| Tested Species Reactivity | Fruit fly |
|---|---|
| Predicted Species Reactivity | Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish |
| Product Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Application | WB |
| Reconstitution and Storage | For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Immunogen | The immunogen is a synthetic peptide corresponding to a region of Fruit fly |
| Purification | Protein A purified |
| Predicted Homology Based on Immunogen Sequence | Cow: 93%; Dog: 93%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 93%; Rabbit: 93%; Rat: 100%; Zebrafish: 93% |
| Complete computational species homology data | Anti-exd (ARP47897_T100) |
| Peptide Sequence | Synthetic peptide located within the following region: EAKEELARKCGITVSQVSNWFGNKRIRYKKNIGKAQEEANLYAAKKAAGA |
| Concentration | Batch dependent within range: 100 ul at 0.5 - 1 mg/ml |
| Blocking Peptide | For anti-exd (ARP47897_T100) antibody is Catalog # AAP47897 (Previous Catalog # AAPP27341) |
| Datasheets/Manuals | Printable datasheet for anti-exd (ARP47897_T100) antibody |
| Gene Symbol | exd |
|---|---|
| Official Gene Full Name | Extradenticle |
| Alias Symbols | Dmel_CG8933, CG8933, DExd, Dm-EXD, Dpbx, EXD, td48, unnamed, anon-EST:fe1H3, DmelCG8933, Exd, l(1)IV, Pbx1 |
| NCBI Gene Id | 32567 |
| Protein Name | Homeobox protein extradenticle |
| Description of Target | As a transcription factor, exd acts with the selector homeodomain proteins altering the regulation of downstream target genes such as wingless, teashirt and decapentaplegic. Thus exd affects segmental identity. |
| Swissprot Id | P40427 |
| Protein Accession # | NP_727924 |
| Nucleotide Accession # | NM_167479 |
| Protein Size (# AA) | 376 |
| Molecular Weight | 42kDa |
| Tissue Tool | Find tissues and cell lines supported by DNA array analysis to express exd.  |
| RNA Seq | Find tissues and cell lines supported by RNA-seq analysis to express exd.  |
| Protein Interactions | CG31957; CG18764; Fer2LCH; CG6454; fzo; ATPsyn-d; hth; CG7443; Dfd; Sec8; CG32425; CG32176; tamo; RpS11; en; nito; E2f2; CG13962; CG7231; Rack1; sip2; Taf12L; dod; Bap60; CG2972; CG12661; MED22; ftz; |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
- IHC Tips & Tricks
- ICC Tips & Tricks
- ELISA Tips & Tricks
- WB/IB Tips & Tricks
- IP Tips & Tricks
See our General FAQ page.
- Reviewed Data:
- Product Protocols: exd antibody tested with Human Drosophila (ARP47897_T100)
What is the species homology for "exd Antibody - C-terminal region (ARP47897_T100)"?
The tested species reactivity for this item is "Fruit fly". This antibody is predicted to have homology to "Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish".
How long will it take to receive "exd Antibody - C-terminal region (ARP47897_T100)"?
This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".
What buffer format is "exd Antibody - C-terminal region (ARP47897_T100)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.".Additional format options may be available. For more information please contact info@avivasysbio.com.
What are other names for "exd Antibody - C-terminal region (ARP47897_T100)"?
This target may also be called "Dmel_CG8933, CG8933, DExd, Dm-EXD, Dpbx, EXD, td48, unnamed, anon-EST:fe1H3, DmelCG8933, Exd, l(1)IV, Pbx1" in publications.
What is the shipping cost for "exd Antibody - C-terminal region (ARP47897_T100)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
What is the guarantee for "exd Antibody - C-terminal region (ARP47897_T100)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
Can I get bulk pricing for "exd Antibody - C-terminal region (ARP47897_T100)"?
You can get bulk pricing for this item by going here.
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "42kDa".Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.
What protocols are available for "exd Antibody - C-terminal region (ARP47897_T100)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
What are positive controls for "EXD"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What are negative controls for "EXD"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What other proteins interact with "EXD"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
What biological processes are associated with "EXD"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
What cellular components are associated with "EXD"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
What protein functions are associated with "EXD"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
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当然跟物种有关。
【GAPDH】或G3PDH是甘油醛-3-磷酸脱氢酶（ glyceraldehyde-3-phosphate dehydrogenase ）的英文缩写。GAPDH是参与糖酵解的一种关键酶，由4个30-40kDa的亚基组成，分子量146kDa，检测条带大约在36kDa。GAPDH基因几乎在所有组织中都高水平表达，且GAPDH 作为管家基因在同种细胞或者组织中的蛋白质表达量一般是恒定的。
【Western Blot】蛋白质印迹法（免疫印迹试验）。它是分子生物学、生物化学和免疫遗传学中常用的一种实验方法。其基本原理是通过特异性抗体对凝胶电泳处理过的细胞或生物组织样品进行着色。通过分析着色的位置和着色深度获得特定蛋白质在所分析的细胞或组织中表达情况的信息。
一般可与大鼠、小鼠、人、兔均有交叉反应，即可作为上述任一来源蛋白的内参。
Actin分子量为42kD，为肌动蛋白
GAPDH分子量为36kD，为磷酸脱氢酶
内参抗体：
Beta-Actin mAb (1C7) 细胞总蛋白 42 kD Abbkine A01010 小鼠源单克隆
Beta-Actin mAb (1C7) , HRP 细胞总蛋白 42 kD Abbkine A01015 小鼠源单克隆,HRP偶联
GAPDH mAb (2B5) 细胞总蛋白 36 kD Abbkine A01020 小鼠源单克隆
GAPDH mAb (2B5) , HRP 细胞总蛋白 36 kD Abbkine A01025 小鼠源单克隆,HRP偶联
Beta-Tubuline mAb (3G6) 细胞总蛋白 55 kD Abbkine A01030 小鼠源单克隆
Plant Actin mAb (3T3) 植物细胞总蛋白 42 kD Abbkine A01050 小鼠源单克隆
PCNA mAb (1D7) 细胞核蛋白 28 kD Abbkine A01040 小鼠源单克隆
COX IV mAb (14Y2) 细胞线粒体总蛋白 16 kD Abbkine A01060 小鼠源单克隆
Histone H3 mAb (2D10) 细胞核蛋白 18 kD Abbkine A01070 小鼠源单克隆
标签抗体（Tag Antibody）可用于检测各种商品化表达载体上的标签序列（如：Myc、Flag、His、GST、HA等），籍以分析目的蛋白的表达含量及其功能；
标签抗体：
Anti-Biotin Antibodies
Anti-Dye Antibodies
Anti-FITC Antibodies
Anti-Fluorescent Protein Antibodies
Anti-HRP Antibodies
Beta Galatosidase Antibodies
FLAG Tag Antibodies
GST Tag Antibodies
HA Tag Antibodies
His Tag Antibodies
Myc Tag Antibodies
TAP Tag Antibodies
V5 Tag Antibodies
因为这个实验用到了抗体，抗体是特异识别某一种蛋白的，所以只要能检测到信号，说明样品中有该蛋白存在，如果有相应的对照，也能半定量说明该蛋白的量如何。
