

Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.

ThisantibodyisshippedwithitsantigenFREEofcharge!
- Peptide(C)HLVEVVPNGDLVRRD,correspondingtoaminoacidresidues201-215ofratCALCR(Accession P32214).
- Westernblotanalysisofratbrainmembrane(lanes1and3)andmousebrainlysate(lanes2and4):1,2. Anti-CalcitoninReceptor(extracellular) Antibody(#ACR-053),(1:200).
3,4.Anti-CalcitoninReceptor(extracellular)Antibody,preincubatedwiththenegativecontrolantigen.
- ExpressionofCALCRinratPC12cellsCellsurfacedetectionof CALCRinintactlivingratpheochromocytoma(PC12)cells.A,C.Extracellularstainingofcellsusing Anti-CalcitoninReceptor(extracellular) Antibody,(#ACR-053),(1:50).B,D.MergedimagesofAandC,eachwithitsrespectiveliveviewofthecells.
- 1.Segovia-Silvestre,T.etal.(2011)BMCRes.Notes4,407.
- 2.Barwell,J.etal.(2012)Br.J.Pharmacol.166,51.
- 3.Taboulet,J.etal.(1998)Hum.Mol.Genet.7,2129.
- 4.Gorn,A.H.etal.(1995)J.Clin.Invest.95,2680.
- 5.Gorn,A.H.etal.(1992)J.Clin.Invest.90,1726.
- 6.Gooi,J.H.etal.(2014)J.Endocrinol.221,181.
thecalcitoninreceptor(CTR)isaclassB G-proteincoupledreceptor (GPCR)thatsignalsviaactivationofcAMPandproteinkinaseC(PKC).Itisoneof15humanclassBGPCRs1,2.ApartfromCTR,calcitoninreceptor-likereceptor(CTLR)istheonlyotherB-classGPCRtobindcalcitonin.Todate,therearethreeknownhumanCTRmRNAisoformsarisingfromalternativesplicing3.
Calcitoninitselfisapeptidehormonethatcontributestobonecalcificationbydirectinhibitionofosteoclastswhichresorbthebonytissue.Itispartofasix-memberfamilythathasvariedfunctionalities.Bybindingtoreceptoractivitymodifyingproteins(RAMPs),CTRandCTLRcanformreceptorstoeverymemberofthecalcitoninfamily.Forinstance,RAMP1andCTRconstructthecalcitoningene-relatedpeptide(CGRP)receptor.
BothCTRandCTLRhaveseventransmembranedomains,anintracellularC-terminal,andanextracellularN-terminal.Toactivatethereceptors,theirN-terminalmustfirstcapturetheC-terminaloftheligandandthencarriedtothetransmembraneloopswhereitbestowsaconformationalchangeuponthem.
Besidesthebone,CTRhasprominentrolesinthekidney,CNS,placenta,ovaries,sperm,lung,andinthebrain4,5.
Inosteoclasts,theexpressionofCTRsdeclineswithage.Interestingly,polymorphismoftheCTRgeneisstronglyassociatedwithdecreasedfracture-riskinpost-menopausalwomen3,6.
AlomoneLabsispleasedtoofferahighlyspecificantibodydirectedagainstanepitopeofratCT-R.Anti-CalcitoninReceptorAntibody(#ACR-053)canbeusedinwesternblotandlivecellimagingapplications.IthasbeendesignedtorecognizeCT-Rfromhuman,ratandmousesamples.
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β-Actin作为内参是得到了公认的,这是针对大多数组织和细胞来说的,它广泛分布于细胞浆内,表达量非常丰富。Beta-actin由375个氨基酸组成,分子量大小为42-43kDa左右。
β-actin的蛋白水平通常不会发生改变,因此被广泛用于Western时上样量是否一致的参照,也常被用于免疫染色观察细胞的微丝结构。在用作Western的参照时,Actin抗体和Tubulin抗体的主要不同之处在于两者所识别蛋白的分子量不同,这样可以选择合适的参照在同一块胶同一张膜上实现同时检测目标蛋白和参照蛋白。向左转|向右转
当然跟物种有关。
因为内参有好几种,分子量差别也比较大,更换分子量与检测蛋白差距更大的内参就可以避免这个问题
另外也可以先用一抗孵育显色和检测,再用Strip缓冲液洗掉膜上的抗体,重新进行内参的抗体孵育显色检测。这样也可以将检测蛋白和内参显色在同一张膜上
因为这个实验用到了抗体,抗体是特异识别某一种蛋白的,所以只要能检测到信号,说明样品中有该蛋白存在,如果有相应的对照,也能半定量说明该蛋白的量如何。
虽然没有具体用过检测这个抗原的抗体,但是如果希望抗体特异性好点,能检测丰度低的蛋白,还是选Abcam吧,Santa有时候全凭人品了,内参抗体什么的还能凑活,其他的比较勉强。
虽然没有具体用过检测这个抗原的抗体,但是如果希望抗体特异性好点,能检测丰度低的蛋白,还是选Abcam吧,Santa有时候全凭人品了,内参抗体什么的还能凑活,其他的比较勉强。

