Description
C1 Inhibitor Polyclonal Antibody – HRP Conjugated
Affinity’s C1 Inhibitor Polyclonal Antibody – HRP Conjugated is the base level of our horseradish peroxidase conjugated C1 Inhibitor antibodies. The purity of IgG is typically 90% and is provided in a solution of HEPES buffered saline containing 50% glycerol (v/v). The titre is essentially the same as the starting antiserum and each vial typically contains the amount of IgG recovered from one milliliter of antiserum however this IgG has been conjugated with Horseradish Peroxidase as an enzyme reporter. This C1 Inhibitor Polyclonal Antibody – HRP Conjugated is generally intended for use as labeled primary antibodies in applications such as immunoassay and immunoblotting.
Product Code: GACINH-HRP
Retail Product Size: 0.2mg vial
Host Animal: Goat Anti-Human C1 Inhibitor Polyclonal Antibody – HRP Conjugated
Species Cross Reactivity: View Chart
Product Datasheet: C1-Esterase Inhibitor Polyclonal Antibody, HRP conjugated purified anti-human Goat IgG
C1 Inhibitor Description
C1 Inhibitor (C1-INH), also known as C1 Esterase Inhibitor, is a part of the serpin family of proteases – alpha-1-antitrypsin (A1AT) and antithrombin (ATIII). These proteins together inactivate target proteases and produce complexes with the protein that is to be inhibited. Cl Inhibitor is the major plasma inhibitor of activated Hageman factor (Factor XII) and kallikrein¹. C1-INH also blocks activation of C1 and the classic complement pathway, without C1-INH the activation of C1, C2, and C4 occur before other inhibitors like C4b-binding protein (C4BP) and Complement Factor I or C3b/C4b inactivator can halt the cascade.
References and Reviews
- Kaplan AP, Ghebrehiwet B, Silverberg M, Sealey JE; The intrinsic coagulation-kinin pathway, complement cascades, plasma renin-angiotensin system, and their interrelationships; Crit Rev Immunol 1981 Sep;3(1):75-93.
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所以免疫组化在组织定位和定性上比较准确，但是在定量上不够准确。
western blot也是利用抗体和抗原之间的结合具有高度的特异性。经过聚丙烯酰胺凝胶电泳分离的蛋白质样品，转移到固相载体上，固相载体以非共价键形式吸附蛋白质，且能保持电泳分离的多肽类型及其生物学活性不变。以固相载体上的蛋白质或多肽作为抗原，与对应的抗体起免疫反应，再与酶或同位素标记的第二抗体起反应。
western blot因为有内参标定，所以在定量上要更加准确。但是在组织定位上不如免疫组化
虽然没有具体用过检测这个抗原的抗体，但是如果希望抗体特异性好点，能检测丰度低的蛋白，还是选Abcam吧，Santa有时候全凭人品了，内参抗体什么的还能凑活，其他的比较勉强。
虽然没有具体用过检测这个抗原的抗体，但是如果希望抗体特异性好点，能检测丰度低的蛋白，还是选Abcam吧，Santa有时候全凭人品了，内参抗体什么的还能凑活，其他的比较勉强。
当然跟物种有关。
β-Actin作为内参是得到了公认的，这是针对大多数组织和细胞来说的，它广泛分布于细胞浆内，表达量非常丰富。Beta-actin由375个氨基酸组成，分子量大小为42-43kDa左右。
β-actin的蛋白水平通常不会发生改变，因此被广泛用于Western时上样量是否一致的参照，也常被用于免疫染色观察细胞的微丝结构。在用作Western的参照时，Actin抗体和Tubulin抗体的主要不同之处在于两者所识别蛋白的分子量不同，这样可以选择合适的参照在同一块胶同一张膜上实现同时检测目标蛋白和参照蛋白。向左转|向右转
