Polyclonal Antibody to Erythropoietin (EPO)
EP; Epoetin; Erythropoetin; Hematopoietin; Hemopoietin
- Product No.PAA028Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig TypeIgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- ImmunogenRPA028Ra01-Recombinant Erythropoietin (EPO)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IP.If the antibody is used in flow cytometry, please check FCM antibodies.
- DownloadInstruction Manual
- UOM20µl100µl200µl1ml10ml
- FOBUS$ 103 For more details, please contact local distributors!US$ 239 For more details, please contact local distributors!US$ 342 For more details, please contact local distributors!US$ 855 For more details, please contact local distributors!US$ 3420 For more details, please contact local distributors!
SPECIFITY of the Polyclonal Antibody to Erythropoietin (EPO)
The antibody isa rabbit polyclonal antibody raised against EPO. It has been selected for its ability to recognize EPO in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Erythropoietin (EPO)
Western blotting: 0.5-2ug/mlImmunocytochemistry in formalin fixed cells: 5-20ug/mlImmunohistochemistry in formalin fixed frozen section: 5-20ug/mlImmunohistochemistry in paraffin section: 5-20ug/mlEnzyme-linked Immunosorbent Assay: 0.05-2ug/mlOptimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Erythropoietin (EPO)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Erythropoietin (EPO)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized ServiceProtein A/G Purification ColumnStaining Solution for Cells and TissuePositive Controlfor AntibodyTissue/Sections Customized ServicePhosphorylated Antibody Customized ServiceWestern Blot (WB) Experiment ServiceImmunohistochemistry (IHC) Experiment ServiceImmunocytochemistry (ICC) Experiment ServiceFlow Cytometry (FCM) Experiment ServiceImmunoprecipitation (IP) Experiment ServiceImmunofluorescence (IF) Experiment ServiceBufferDAB Chromogen KitSABC KitLong-arm Biotin Labeling KitReal Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Rattus norvegicus (Rat) | Applications (RESEARCH USE ONLY!) |
| APA028Ra61 | Active Erythropoietin (EPO) | Cell culture; Activity Assays. |
| EPA028Ra61 | Eukaryotic Erythropoietin (EPO) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA028Ra01 | Recombinant Erythropoietin (EPO) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA028Ra01 | Polyclonal Antibody to Erythropoietin (EPO) | WB; IHC; ICC; IP. |
| MAA028Ra21 | Monoclonal Antibody to Erythropoietin (EPO) | WB; IHC; ICC; IP. |
| SEA028Ra | ELISA Kit for Erythropoietin (EPO) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA028Ra | CLIA Kit for Erythropoietin (EPO) | Chemiluminescent immunoassay for Antigen Detection. |
| KSA028Ra01 | ELISA Kit DIY Materials for Erythropoietin (EPO) | Main materials for "Do It (ELISA Kit) Yourself". |
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虽然没有具体用过检测这个抗原的抗体,但是如果希望抗体特异性好点,能检测丰度低的蛋白,还是选Abcam吧,Santa有时候全凭人品了,内参抗体什么的还能凑活,其他的比较勉强。
我觉得封闭这一步时间长短多少并不能减少背景…背景过深多半还是抗体浓度过大…不管一抗还是二抗过大都会使背景加深…一抗的浓度不一定是条带清晰就是最佳了…联系进一步优化一下
这个主要还是抗体的问题,WB最主要的还是要选择一个特异性较好的抗体,抗体是整个实验的重中之重
虽然没有具体用过检测这个抗原的抗体,但是如果希望抗体特异性好点,能检测丰度低的蛋白,还是选Abcam吧,Santa有时候全凭人品了,内参抗体什么的还能凑活,其他的比较勉强。
以上信息来自。
因为这个实验用到了抗体,抗体是特异识别某一种蛋白的,所以只要能检测到信号,说明样品中有该蛋白存在,如果有相应的对照,也能半定量说明该蛋白的量如何。


