CLIA Kit for Fibroblast Growth Factor 13 (FGF13)
FHF2; Fibroblast Growth Factor Homologous Factor 2
- Product No.CCC915Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range6.17-500pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 2.42pg/mL.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 588 For more details, please contact local distributors!US$ 840 For more details, please contact local distributors!US$ 3780 For more details, please contact local distributors!US$ 7140 For more details, please contact local distributors!US$ 58800 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- Typical Standard Curve
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the CLIA Kit for Fibroblast Growth Factor 13 (FGF13)
This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 13 (FGF13).No significant cross-reactivity or interference between Fibroblast Growth Factor 13 (FGF13) and analogues was observed.
Recovery of the CLIA Kit for Fibroblast Growth Factor 13 (FGF13)
Matrices listed below were spiked with certain level of recombinant Fibroblast Growth Factor 13 (FGF13) and the recovery rates were calculated by comparing the measured value to the expected amount of Fibroblast Growth Factor 13 (FGF13) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 96-104 | 101 |
| EDTA plasma(n=5) | 97-104 | 101 |
| heparin plasma(n=5) | 97-104 | 101 |
Precision of the CLIA Kit for Fibroblast Growth Factor 13 (FGF13)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 13 (FGF13) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 13 (FGF13) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the CLIA Kit for Fibroblast Growth Factor 13 (FGF13)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fibroblast Growth Factor 13 (FGF13) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-95% | 79-104% | 94-103% | 85-101% |
| EDTA plasma(n=5) | 79-102% | 99-105% | 79-101% | 94-103% |
| heparin plasma(n=5) | 81-93% | 89-101% | 93-101% | 84-98% |
Stability of the CLIA Kit for Fibroblast Growth Factor 13 (FGF13)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the CLIA Kit for Fibroblast Growth Factor 13 (FGF13)
1. Prepare all reagents, samples and standards;2. Add 50µL standard or sample to each well.And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C;3. Aspirate and wash 3 times;4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;5. Aspirate and wash 5 times;6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;7. Read RLU value immediately.
Test principle of the CLIA Kit for Fibroblast Growth Factor 13 (FGF13)
The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Fibroblast Growth Factor 13 (FGF13). A competitive inhibition reaction is launched between biotin labeled Fibroblast Growth Factor 13 (FGF13) and unlabeled Fibroblast Growth Factor 13 (FGF13) (Standards or samples) with the pre-coated antibody specific to Fibroblast Growth Factor 13 (FGF13). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Fibroblast Growth Factor 13 (FGF13) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Fibroblast Growth Factor 13 (FGF13) level in the sample or standard.
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Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| APC915Hu02 | Active Fibroblast Growth Factor 13 (FGF13) | Cell culture; Activity Assays. |
| RPC915Hu02 | Recombinant Fibroblast Growth Factor 13 (FGF13) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC915Hu01 | Recombinant Fibroblast Growth Factor 13 (FGF13) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC915Hu01 | Active Fibroblast Growth Factor 13 (FGF13) | Cell culture; Activity Assays. |
| CPC915Hu21 | OVA Conjugated Fibroblast Growth Factor 13 (FGF13) | Immunogen; SDS-PAGE; WB. |
| PAC915Hu08 | Polyclonal Antibody to Fibroblast Growth Factor 13 (FGF13) | WB; IHC; ICC; IP. |
| PAC915Hu01 | Polyclonal Antibody to Fibroblast Growth Factor 13 (FGF13) | WB; IHC; ICC; IP. |
| PAC915Hu02 | Polyclonal Antibody to Fibroblast Growth Factor 13 (FGF13) | WB; IHC; ICC; IP. |
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| KSC915Hu11 | ELISA Kit DIY Materials for Fibroblast Growth Factor 13 (FGF13) | Main materials for "Do It (ELISA Kit) Yourself". |
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所以免疫组化在组织定位和定性上比较准确，但是在定量上不够准确。
western blot也是利用抗体和抗原之间的结合具有高度的特异性。经过聚丙烯酰胺凝胶电泳分离的蛋白质样品，转移到固相载体上，固相载体以非共价键形式吸附蛋白质，且能保持电泳分离的多肽类型及其生物学活性不变。以固相载体上的蛋白质或多肽作为抗原，与对应的抗体起免疫反应，再与酶或同位素标记的第二抗体起反应。
western blot因为有内参标定，所以在定量上要更加准确。但是在组织定位上不如免疫组化
当然跟物种有关。
以上信息来自。
高保真生物技术有限公司~实验整体外包服务商~
因为内参有好几种，分子量差别也比较大，更换分子量与检测蛋白差距更大的内参就可以避免这个问题
另外也可以先用一抗孵育显色和检测，再用Strip缓冲液洗掉膜上的抗体，重新进行内参的抗体孵育显色检测。这样也可以将检测蛋白和内参显色在同一张膜上
