
PTEN (Phospho-Ser370) AntibodyPDF
Catalog Number:
E011062-1Amount:
50μg/50μlForm of Antibody:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Storage/Stability:
Store at -20℃/1 yearImmunogen:
The antiserum was produced against synthesized phosphopeptide derived from human PTEN around the phosphorylation site of serine 370 (D-V-SP-D-N).Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.Specificity/Sensitivity:
PTEN (phospho-Ser370) antibody detects endogenous levels of PTEN only when phosphorylated at serine 370.Reactivity:
Human, Mouse, RatApplications:
WB: 1:500~1:1000 IHC: 1:50~1:100Swiss-Prot No.:
P60484References:
Al-Khouri AM, et al. (2005). J Biol Chem.280 (42):35195-35202.Miller SJ, et al. (2002). FEBS Lett. 528(1-3): 145-153.Torres J, et al. (2001). J Biol Chem.276 (2): 993-998.Vazquez F, et al. (2000). Mol Cell Biol.20 (14): 5010-5018.P-Peptide - +Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using PTEN (phospho-Ser370) antibody (E011062).
Western blot analysis of extracts from HeLa cells using PTEN (phospho-Ser370) antibody (E011062).
Price:
145$Research Area:
Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells
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选择Flag标签抗体,主要根据实验的需要。一个抗体检测后应用类型越多,使用中选择余地就越大。要综合考虑抗体来源,特异性,稳定性,效价等等因素
百度上的,,,,,搬运
想做个蛋白的定位,可是没有好抗体,就想做个转基因小鼠,在基因后面加一个标签。但是之前没有相关经验,不知道哪一种tag更好呢,更适合在小鼠里表达呢?因为我蛋白比较小,加GFP那么大的话可能会影响表达。比较小的tag例如HA,Flag还有His等,不知道哪个更适合一些?求大神们给些经验。
但是His标签是目前最普及最方便的融合标签,所以His标签的抗体,不管是单抗还是多抗,鼠源还是兔源都是商品化程度非常高的的抗体。这样的话因为抗体原因失败的可能性就非常小了。
例如 GST (1-109):
•epitope corresponding to amino acids 1-109 mapping at the N-terminus of GST of Schistosoma japonicum origin
•recommended for detection of GST fusion proteins and glutathione-S-transferase (GST) of Schistosoma japonicum origin by WB, IP, IF and ELISA
还有Schistosoma japonicum origin 跟我载体pGEX4T-1表达的融合蛋白可用Western Blot吗?它们有什么关系?
十分感谢

