Host/IsotypeMouse / IgG1
Clone19C4B2.1
Species ReactivityAll species
Validation DataAc-K Antibody White Paper
AAC03, anti-acetyl lysine antibody is a pan-acetyl lysine mouse monoclonal antibody that is part of the Signal-Seeker™ product line.The Anti-acetyl-lysine antibody recognizes proteins post-translationally modified by acetylation on the epsilon amine groups of lysine residues that occur on 30-50% of all proteins and in particular histones, p53, tubulin and myosin. A proprietary mixture of acetylated proteins was used to produce a highly robust antibody that has been shown to recognize a wide range of acetylated proteins in IP, WB, ChIP and IF applications. This Anti-acetyl-lysine antibody has many advantages when compared to other commercially available antibodies as shown below.
Western Blot using Acetyl-Lysine Antibody (AAC03)
Fig 1: A: Murine tissue extract, 30 μg brain extract. B: 30 μg of Cos-7 cell lysate treated with TSA and nicotinamide (+) or untreated (-). Strongly enhanced bands at 55 and 14-16 kDa in TSA-treated lysate correspond to acetylated tubulin and histone proteins, respectively. C: Titration of acetylated BSA. Lanes 1-5 contain 0.5, 0.1, 0.05, 0.01, and 0.005 ng Ac-BSA, lanes 6-7 contain 500 and 1000 ng non-acetylated BSA, respectively. AAC03 was used at a 1:500 dilution following the recommended western blot protocol.
To see the full Western blot comparison, see the Optimized Protocols or the product datasheet.
Immunoprecipitation using Acetyl-Lysine Antibody
Fig 2: Cos-7 cells were either treated (+) or untreated (-) with TSA (1 μM) and nicotinamide (1 mM) for 6 hours. Cell lysates were prepared in BlastR buffer and filter system and 1 mg of lysate per reaction was used for IP of acetylated proteins. 20 ml of AAC03 was used per IP reaction. Western blots of immunoprecipitated proteins were developed using AAC03-HRP at 1:3000 dilution.
To see the full Immunoprecipitation comparison, see the Optimized Protocols or the product datasheet.
Fig 3: Swiss 3T3 cells, untreated (a and c) or treated (b and d) with TSA (1 μM for 6 h), were stained as described in the datasheet. Acetylated proteins were visualized using a green fluorescent secondary. Actin fibers were visualized using a red Rhodamine Phalloidin and the nucleus was stained with DAPI. The acetylated microtubule network is clearly visible with TSA-treatment, while the green fluorescent nuclear intensity indicate the high abundance of acetylated proteins in the nucleus. In c and d, acetylated BSA (10 mg/ml) was used to compete for AAC03 binding as an indicator of AAC02 specificity for acetyl-lysine modifications.
To see the full Immunofluorescence protocol, see the Optimized Protocols or the product datasheet.
Fig 4: Utilization of AAC03 for ChIP. Chromatin was prepared from A431 cells, either untreated or treated with TSA (1 μM) and nicotinamide (1 mM) for 6 hours. ChIP was performed as described. mIgG: mouse IgG used for ChIP control; AAC02: anti-acetyl lysine antibody used for ChIP; Input: cell lysate prior to ChIP; H2O: Water used as PCR control. The PCR products obtained with GAPDH primers are 166 bp.
To see the recommended ChIP protocol, see the Optimized Protocols or the product datasheet.
Acetylation of proteins can occur as a co-translational or post-translational modification (PTM) (1). Co-translational acetylation occurs at the N-terminal of approximately 85% of mammalian proteins, it is irreversible and is thought to be important in protein stability, localization and synthesis (1). Post-translational acetylation occurs on the epsilon amino group of lysine residues as a reversible and highly dynamic PTM that is known to be a key regulator in multiple cellular events, including chromatin structure, transcription, metabolism, signal transduction and cytoskeletal regulation (2-3). To date over 4,000 proteins have been identified as targets for PTM acetylation which is comparable to phosphorylation in cellular prevelance (3). Antibody AAC01 detects acetyl lysine PTMs.
References
1 Bogdan P. and Sherman F. 2002. The diversity of acetylated proteins. Genome Biol. 3 (5): reviews 0006.
2 Lundby A. et al. 2012. Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and cellular patterns. Cell Reports 2:419-431.
3 Sadoul K. et al. 2010. The tale of protein lysine acetylation in the cytoplasm. J. Biomed. Biotech. 2011:1-15.
4 Golemis EA et. Al, Protein-Protein Interactions, CSHLP, 2005, p67
For more information contact: signalseeker@cytoskeleton.com
Associated Products:
Signal-Seeker™ Acetyl-Lysine Detection Kit (Cat. # BK163)
Signal-Seeker™: BlastR™ Rapid Lysate Prep Kit (Cat. # BLR01)
Signal-Seeker™ Acetylation Affinity Beads (Cat.# AAC04-beads)
For product Datasheets, MSDSs, and COAs please click on the PDF links below.
Sample Size Datasheet (Cat. AAC03-s): 
Certificate of Analysis: available upon request
For the most recent publications citing this and other Signal-Seeker™ products, see our Signal-Seeker™ Validation Data Page click here
Visit our Signal-Seeker™ Tech Tips and FAQs page for technical tips and frequently asked questions regarding this and other Signal-Seeker™ products click here
If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com
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以下来自摆渡百科
细胞在发生凋亡时，会激活一些DNA内切酶，这些内切酶会切断核小体间的基因组DNA。细胞凋亡时抽提DNA进行电泳检测，可以发现180-200bp的DNA ladder。基因组DNA断裂时，暴露的3’-OH可以在末端脱氧核苷酸转移酶(Terminal
Deoxynucleotidyl Transferase, TdT) 的催化下加上荧光素 (FITC) 标记的dUTP
(fluorescein-dUTP) ，从而可以通过荧光显微镜或流式细胞仪进行检测，这就是TUNEL (TdT-mediated dUTP
Nick-End Labeling) 法检测细胞凋亡的原理。
第二个问题：兔子不能免疫兔子的。免疫的一个重要概念是识别“自己”和“非己”，如果对自己的蛋白产生免疫反应，那就麻烦了。
可以类比器官移植，亲缘关系越近，越不容易产生免疫排斥。
sc-153是兔抗大鼠ERK2的多克隆抗体
还有很多关于ERK1和ERK2的抗体
若想知道更多信息，你可以拨打Santa cruz 上海分公司的电话咨询
021-6093-6351
我们会按照你的实验需求推荐最适合你的产品。
你看这个抗体的质量怎么样，说明里面有没有说可以做组化，还是只能做western blot。
想买消炎药怎么办?
去药店买药想买不是抗生素的消炎药，那工作人员说不是抗生素怎么消炎呢，请问知情网友求一替代品，谢了。
