Host/IsotypeMouse/IgG2b Clone3C6.08.20 SpeciesReactivityAllspecies | Anti-acetyllysineantibodyisapan-acetyllysinemousemonoclonalantibodythatispartoftheSignal-Seeker™productline.TheAnti-acetyl-lysineantibodyrecognizesproteinspost-translationallymodifiedbyacetylationontheepsilonaminegroupsoflysineresiduesthatoccuron30-50%ofallproteinsandinparticularhistones,p53,tubulinandmyosin.AproprietarymixtureofacetylatedproteinswasusedtoproduceahighlyrobustantibodythathasbeenshowntorecognizeawiderangeofacetylatedproteinsinIP,WB,ChIPandIFapplications.ThisAnti-acetyl-lysineantibodyhasmanyadvantageswhencomparedtoothercommerciallyavailableantibodiesasshownbelow.
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ValidatedApplications
WesternBlotusingAcetylLysineAntibody Cytoskeleton"sAnti-AcetylLysineAntibodyCat.#AAC01recognizes0.005ngofchemicallyacetylatedBSAandiscomparableinsensitivitytoothercommerciallyavailableantibodies.UnlikeotherAnti-AcetyleLysineantibodiesthisantibodydoesnotshowcrossreactivitywithnon-acetylatedBSA.ToseethefullWesternblotcomparison,seetheOptimizedProtocolsortheproductdatasheet. |  |
ImmunoprecipitationusingAcetylLysineAntibody ABIlityofAAC01toIPhistoneswascomparedtoothercommerciallyavailableantibodies.Cytoskeleton"sAnti-AcetylLysineAntibodyprovidesclearadvantagesforIPapplications.Eachtubeissufficientforapproximately20IPassays.ToseethefullImmunoprecipitationcomparison,seetheOptimizedProtocolsortheproductdatasheet. |  |
ImmunofluorescenceusingAcetylLysineAntibody HumanepidermoidcarcinomaA431cells,untreated(top)ortreated(bottom)withTSA(5mMfor12h),werestainedasdescribedinthemethod.Acetylatedcytoplasmicandnuclearproteinswerevisualizedingreenfluorescence.Notethatincontrastwiththeuntreatedcontrol,acetylatedmicrotubulenetworkisclearlyvisIBLeinTSA-treatedsample.Thefluorescentnuclearintensitiesindicatethehighabundanceofacetylatedproteinsinthenucleus.ToseethefullImmunofluorescenceprotocol,seetheOptimizedProtocolsortheproductdatasheet. |  |
ChIPusingAcetylLysineAntibody ChromatinwaspreparedfromA431cells,eitheruntreatedorTSA-treated(5mM,4hrs).Briefly,cellswerefixedwith1%formaldehydefor10minandenzymatically-shearedchromatinswereimmunoprecipitatedbyusinganti-acetylantibodies(1:100dilution).ThepromoterregionofhousekeepinggeneGAPDHwasamplifiedbyaprimerpairandPCRproductswereanalyzedby2%agarosegel-electrophoresis.ToseetherecommendedChIPprotocol,seetheOptimizedProtocolsortheproductdatasheet. |  |
ProteinAcetylationBackground
Acetylationofproteinscanoccurasaco-translationalorpost-translationalmodification(PTM)(1).Co-translationalacetylationoccursattheN-terminalofapproximately85%ofmammalianproteins,itisirreversibleandisthoughttobeimportantinproteinstability,localizationandsynthesis(1).Post-translationalacetylationoccursontheepsilonaminogroupoflysineresiduesasareversibleandhighlydynamicPTMthatisknowntobeakeyregulatorinmultiplecellularevents,includingchromatinstructure,transcription,metabolism,signaltransductionandcytoskeletalregulation(2-3).Todateover4,000proteinshavebeenidentifiedastargetsforPTMacetylationwhichiscomparabletophosphorylationincellularprevelance(3).AntibodyAAC01detectsacetyllysinePTMs.
References
1BogdanP.andShermanF.2002.Thediversityofacetylatedproteins.GenomeBiol.3(5):reviews0006.
2LundbyA.etal.2012.Proteomicanalysisoflysineacetylationsitesinrattissuesrevealsorganspecificityandcellularpatterns.CellReports2:419-431.
3SadoulK.etal.2010.Thetaleofproteinlysineacetylationinthecytoplasm.J.Biomed.Biotech.2011:1-15.
4GolemisEAet.Al,Protein-ProteinInteractions,CSHLP,2005,p67
Formoreinformationcontact:signalseeker@cytoskeleton.com
AssociatedProducts:
Signal-Seeker™:BlastR™RapidLysatePrepKit(Cat.#BLR01)
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以下来自摆渡百科
细胞在发生凋亡时，会激活一些DNA内切酶，这些内切酶会切断核小体间的基因组DNA。细胞凋亡时抽提DNA进行电泳检测，可以发现180-200bp的DNA ladder。基因组DNA断裂时，暴露的3’-OH可以在末端脱氧核苷酸转移酶(Terminal
Deoxynucleotidyl Transferase, TdT) 的催化下加上荧光素 (FITC) 标记的dUTP
(fluorescein-dUTP) ，从而可以通过荧光显微镜或流式细胞仪进行检测，这就是TUNEL (TdT-mediated dUTP
Nick-End Labeling) 法检测细胞凋亡的原理。
sc-153是兔抗大鼠ERK2的多克隆抗体
还有很多关于ERK1和ERK2的抗体
若想知道更多信息，你可以拨打Santa cruz 上海分公司的电话咨询
021-6093-6351
我们会按照你的实验需求推荐最适合你的产品。
你看这个抗体的质量怎么样，说明里面有没有说可以做组化，还是只能做western blot。
第二个问题：兔子不能免疫兔子的。免疫的一个重要概念是识别“自己”和“非己”，如果对自己的蛋白产生免疫反应，那就麻烦了。
可以类比器官移植，亲缘关系越近，越不容易产生免疫排斥。
